摘要
为了进一步明确家蚕和野桑蚕的亲缘关系,收集了中国和日本的一些家蚕和野桑蚕品种资源,提取了家蚕和野桑蚕的总mRNA,通过RT-PCR克隆了家蚕和野桑蚕线粒体DNA(mtDNA)的细胞色素氧化酶亚基1基因(cytochromeoxidasesubunit1gene,COⅠ)和NADH-6基因,并制备探针进行了DIG-RFLP检测。结果表明,中国和日本的家蚕与中国野桑蚕的COⅠ和NADH-6基因的DIG-RFLP的分子多态性相同,但与日本野桑蚕存在差异。此结果从线粒体水平证实了中国和日本的家蚕都起源于中国野桑蚕,而不是起源于日本的野桑蚕。
To obtain more information about the genetic relationship between domestic and wild silkworms, we collected domestic silkworms （Bombyx mori L. ） and wild silkworms （B. mandarina Moore） from China and Japan, respectively. Total mRNAs of domestic and wild silkworms were extracted, and CO Ⅰ and NADH-6 genes from mtDNA were cloned by RT-PCR and analysed by DIG-RFLP method. The results showed that the Chinese domestic silkworm had the same molecular diversity as that of the Japanese domestic silkworm and Chinese wild silkworm, but had different diversity from that of the Japanese wild silkworm. The results suggested that both Chinese and Japanese domestic silkworms originated from Chinese wild silkworm but not from Japanese wild silkworm.
出处
《昆虫学报》
CAS
CSCD
北大核心
2006年第3期470-473,共4页
Acta Entomologica Sinica
基金
国家"973"计划资助项目(2005CB121005)
国家自然科学基金项目(30471309)
