摘要
目的用人单核细胞白血病细胞系建立一种可重复的裸鼠中枢神经系统白血病(CNSL)模型。方法对4和6周龄 BALB/c 裸鼠进行切脾,环磷酰胺腹腔注射及全身亚致死剂量照射等预处理(SCI 预处理),经尾静脉接种1×lO^7人单核细胞白血病细胞系 SHI-1细胞,应用 RT-PCR、组织病理切片、免疫组织化学及流式细胞术检测裸鼠各脏器中 SHI-1细胞浸润生长情况。结果裸鼠的生存期为33～46 d ,部分裸鼠于5周后出现双下肢截瘫 SHI-1细胞可浸润多种脏器,并可形成淡绿色肿块,病理示在肝、肺、肾、睾丸等多部位发现有 SHI-1细胞浸润,椎体及颅骨骨髓腔中有大量白血病细胞浸润,并可突破骨髓腔向椎管及颅内浸润,多位于硬膜及蛛网膜下腔,并可由脑皮层表面向深部脑组织浸润。结论 SHI-1细胞可于经 SCI 预处理的裸鼠中形成稳定的、可重复的 CNSL 模型,可用于对 CNSL 的实验研究。
Objective To establish a model of human monocytic leukemia with CNS infiltration in BALB/c nude mice. Methods BALB/c nu/nu mice pre-treated by splenectomy, cytoxan intraperitoneal injection, and sublethal irradiation（SCI）, were transplanted intravenously with 1 × 10^7 of human monocytic leukemic SHI-1 cells. The leukemic cells engrafted in the mice were detected by RT-PCR, histopathological examination, immunohistochemistry and FCM. Results The survival time of SCI-nu/nu mice was 33 -46 d. Paraplegia occurred in some of the mice. 5 weeks after transplantation, SHI-1 cells engrMted in SCI-nu/nu mice, multi-organs were involved and green solid neoplasms were formed in some organs. Histopatilological examination found that SHI-1 cells infihrated in liver, lung, kidney and testis of the mice and vertebral and skull bone marrow was replaced by leukemic cells. Leukemic cell penetrated through the surface of vertehrae, formed neoplasm, and entered the subdural space, but seldom involved the spinal parenehyma. In brain leukemia cells were filled in the subdural space and pia-arachnoid, covered the surface of eerebram, cerebellum, spread along the virchow-robin space on the surface of pia mater, and eventually invaded the brain parenchyma. Conclusion SHI-1 cells could engrafted in the SCI-nu/nu mice, lorm an efficient and reproducible experimental model of CNSL and systematic leukemia. This model may be usefully for studying the pathogenesis of CNSL.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2006年第6期374-378,共5页
Chinese Journal of Hematology
基金
江苏省卫生厅基金(H200327)
