摘要
在ＫＩＯ４存在下，新有机试剂２（－２－苯并噻唑偶氮）－５－磺丙氨基苯酚与Ｃｏ（Ⅲ）和Ｎｉ（Ⅱ）反应形成水溶性、紫红色１∶２配合物而被保留在Ｃ１８硅氧烷键合固定相上，用含有７．５ｍｍｏｌ／Ｌ醋酸铵的甲醇－丙酮－乙醇－水（２７∶１６∶５∶５２，Ｖ／Ｖ）流动相洗脱，在５２５ｎｍ处检测，Ｃｏ（Ⅲ）－和Ｎｉ（Ⅱ）－ＢＴＡＳＰＡＰ配合物在４ｍｉｎ内获得完全分离。最小检测量分别为０．２ｎｇ和０．０９ｎｇ。该方法应用于铝合金试样中钴、镍的分离测定。
In the presence of KIO4, new organic reagent 2-(2-benzothialylazo)-5-(3-sulfopropyl ) aminophenol (H2R) reacts with both Co(Ⅲ) and Ni(Ⅲ) to form water-soluble, purplish chelates. the molar ratios for both Co(Ⅲ) and Ni(Ⅱ) to BTASPAP are 1: 2. The two chelatcs have different retention times on the spherisorb ODS C18 siloxane bonded-phase, as a solution of methanol-acetone-ethanol-water (27: 16: 5: 52. V/V) containing 7. 5 mmol/L NH4Ac is used as eluent. The detection wavelength is 525 nm. The Co(Ⅲ)-and Ni (Ⅱ) BTASPAP chelates could be completely separated in 4 min. The minimum detection amounts for Co and Ni are 0. 2 ng and 0. 09 ng respectively. This method is applied to the determination of trace Co and Ni in aluminum-alloy samples.
关键词
高效液相色谱
显色剂
钴
镍
测定
high performance liquid chromatography
colour reaction reagent
Co
Ni
aluminium-alloy
