摘要
利用100～500 MPa的静水高压和10～30 min的保压时间对粘红酵母（Rh. glutinis RG5）进行处理,发现处理10 min时的高压存活曲线呈现马鞍形,说明高静水压处理的最终效应可能是多种生物学效应的累积.150 MPa保压20 min时的突变株RG5-3的生物量虽然比出发菌株RG5下降了9.25%,但目的产物β-胡萝卜素的质量分数却比RG5提高了68.60%.传代实验表明,该诱变株遗传稳定性良好,没有发生性状退化及回复突变.利用限制性片段长度多态性分析（RFLP）比较发现,限制性内切酶HindⅢ和BamHⅠ切割的RG5和RG5-3的DNA片段有明显不同,证明高静水压使粘红酵母RG5在表面性状和DNA水平上都发生了改变.
In this paper, the β-carotene producing Rh. glutinis RG5 was treated by high hydrostatic pressure （HHP） at 100～500 MPa for 10～30min. The shape of the survival curve for 10min was saddle, which shows that the result of HHP treatment might be due to was the cumulation of many biology effects. The biomass of mutant RG5-3（treated at 150 MPa for 20 min） was decreased 9.25 % compared with that of the untreated RG5, but the relevant β-earotene content was increased 68.60%. Its genetic quality was also proved to be stable by experiments. The DNA fragments cut by Hind Ⅲ and BamH Ⅰ were different, and this result proved that HHP changed Rh. glutinis RG5 in both exterior characters and nucleic acid level.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2006年第3期7-10,24,共5页
Journal of Food Science and Biotechnology
基金
河南省自然科学基金项目(0411021900)
河南省杰出青年基金项目(9902)
关键词
高静水压
粘红酵母
诱变
high hydrostatic pressure （HHP）
Rhodotorula glutinis
mutation
