摘要
目的探讨不同剂量γ射线辐照ACD-全血中红细胞在不同保存期的功能变化。方法将ACD-全血(保质期21d)200ml分为4组,于采血后当天进行0、15、25、35Gyγ射线辐照。所有样品于4℃保存,在辐照后第d0、d7、d14、d21分别测定红细胞三磷酸腺苷(ATP)酶活性、过氧化物岐化酶(SOD)活性、2,3-磷酸甘油酸(2,3-DPG)含量。结果在各保存期,各辐照剂量组的红细胞ATP酶活性、SOD活性、2,3-DPG含量,与对照组比较差异均无显著性(P>0·05),但各组2,3-DPG含量在d7开始下降,d21时已下降为0。结论15-35Gyγ射线辐照对ACD全血的保质期内的红细胞活性(ATP酶)、SOD活性和红细胞携氧能力(2,3-DPG)均无明显影响。
Objective To elucidate the effect of different dosage of gamma irradiation on the function of red blood cells（RBC） in ACD - whole blood at various storage period. Methods ACD - whole blood （expiry dated 21 ） was divided into 4 groups and treated with gamma irradiated at doses of 0 （as control）, 15,25,35Gy on the day of collection. All samples were stored at 4℃ in refrigerator. On the day 0,7,14 and 21, the samples were measured for adenosine triphosphatase （ATP - ase） activity, superoxide dismutase （SOD） activity, 2,3 - diphosphoglyceric acid （2,3 - DPG） content of RBC. Results At various storage period, there no differences in activity of ATP - ase, SOD activity and 2,3 - DPG content were observed in individual irradiated groups at compared with normal controls respectively （ P 〉 0.05）, but 2,3 - DPG content in all irradiated groups and control began to reduce on day 7, and as low as zero on day 21. Conclusion 15 - 35 Gy gamma irradiation does not affect obviously the activity of ATP - ase and SOD activity as well as the oxygen - carrying capacity of RBC （2,3 - DPG） during the period of validity of the ACD - whole blood.
出处
《中国热带医学》
CAS
2006年第6期964-965,977,共3页
China Tropical Medicine
关键词
Γ射线辐照
红细胞
三磷酸腺苷酶
过氧化物岐化酶
2
3-磷酸甘油酸
Gatmma irradtion
Red blood cell
Adenosine triphosphatase , Superoxide dismutase
2,3 - diphosphoglycefic acid
