摘要
目的建立人血浆中奥美拉唑及5-羟奥美拉唑的高效液相色谱分析方法. 方法对羟基苯甲酸乙酯为内标,待测血浆以二氯甲烷提取,并进行高效液相紫外检测,色谱柱为Kromasit C18,5μm,4.6mm×150mm;流动相为0.1mol·L^-1乙酸铵-乙晴-甲醇（55：45：5）,流速1.0 mL·min^-1,检测波长302nm,柱温：室温. 结果血浆中奥美拉唑及5-羟奥美拉唑的线性范围分别为0.005～2.001mg·L^-1和0.02～1.20mg·L^-1,相关系数分别为0.9989和0.9982,相对回收率分别为：93.2%～96.4%,92.5%～97.4%;日内日间变异均小于8%,最低检测限分别为0.005mg·L^-1及0.02mg·L^-1. 结论 HPLC法测定人血浆中奥美拉唑及5-羟奥美拉唑的浓度结果满意,可用于以奥美拉唑为探针药物,分析人群细胞色素氧化酶CYP2C19的表型多态性.
Objective To establish a HPLC method for determining the concentrations of omeprazole and its metabolite 5 - hydroxyomeprazole in human plasma. Methods The internal standard was ethyl p - Hydroxybenzoate. Omeprazole and 5 - hydroxyomeprazole were extracted with diehloromethane and then separated on Kromasit C18（5μm,4.6mm× 150mm） with a mobile phase consisting of 0. 1 mol· L^-1 ammonium acetate ： aeetonitrile： methanol （55：45：5） . The flow rate was 0.1 mol· L-^1. The detection was set on 302nm. Results The calibration curve was linear within 0.005 - 2. 001 mg· L^- 1 for omeprazole and 0.02 - 1.20mg· L^- 1 for 5 - hydroxyomeprazole, The relative recovery rates of omeprazle and 5 - hydroxyomeprazole were 93.2% - 96.4% and 92.5% - 97.4% , respectively. The RSD in a day and the RSD between days were both less than 8% . Conclusion HPLC is s simple, rapid and accurate method for determination of the concentrations of omeprazole and 5 - hydroxyomeprazole in human plasma in addition to the study of phenotype of eytochrome P450 2C19 in Hainan Li ethnic group.
出处
《中国热带医学》
CAS
2006年第6期962-963,共2页
China Tropical Medicine
