摘要
目的应用双向凝胶电泳-质谱法分析正常人及骨关节炎(osteoarthritis,OA)患者滑膜成纤维细胞蛋白质组表达差异,探讨差异蛋白在骨关节炎发病中的作用。方法采用固相pH梯度(immob ilized pH grad ient,IPG)双向凝胶电泳(two-d imensional gel electrophoresis,2-DE)分离正常人及骨关节炎患者滑膜成纤维细胞总蛋白质,银染显色,PDQuest2-DE软件分析图像,比较2种细胞蛋白质组表达差异,对差异蛋白质点用基质辅助激光解析电离飞行时间质谱进行鉴定。结果胶图差异分析显示36个表达水平存在明显差异的蛋白质,选取15个点进行质谱鉴定,鉴定出6个表达上调蛋白质。结论正常人及骨关节炎患者滑膜成纤维细胞表达蛋白质组存在差异。这些差异蛋白可能参与骨关节炎的发病。
Objective To understand the possible roles of some proteins expressed by human synovial fibroblasts in the pathogenesis of osteoarthritis by means of 2-DE and bioinformatics. Methods The difference of synovial fibroblasts protein expression between osteoarthritis patients and healthy controls was analyzed by 2- DE. The differential expression spots were identified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry （MALDI-TOF-MS）, followed by bioinformatics analysis. Results Gel-image analysis revealed that there were 36 differential protein spots. A total of 15 differential protein spots were identified by MALDI-TOF-MS, of which 6 spots were much more intensive in osteoarthritis patients than normal ones. Conclusion These differential proteins based on bioinformatics analysis might be involved in synovitis in osteoarthritis patients.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第11期1221-1223,共3页
Journal of Third Military Medical University
基金
疾病蛋白质组学重庆市市级重点实验室专项基金资助项目(2004)~~
关键词
骨关节炎
滑膜成纤维细胞
蛋白质组
双向电泳
osteoarthritis
synovioblast
proteome
two-dimensional electrophoresis
