摘要
镉是一种有毒重金属,能诱导酿酒酵母的氧化损伤。为了研究低剂量镉诱导细胞氧化损伤的作用,通过有葡萄糖和无葡萄糖的酵母培养基,用浓度2μmol/L和10μmol/L的CdCl2染毒酿酒酵母10h后,统计酿酒酵母细胞的存活率,并用HPLC EC法测定酿酒酵母线粒体DNA中8OH dG/105dG比值。结果表明:无葡萄糖条件下,10μmol/L的CdCl2染毒时酿酒酵母对Cd2+的耐受性比有葡萄糖时明显高(P<0.01),而且线粒体DNA氧化损伤程度也明显低(P<0.01);但2μmol/L的CdCl2染毒时,酿酒酵母对Cd2+的耐受性和线粒体DNA氧化损伤程度与有葡萄糖条件下相比差异不显著(P>0.05)。因此,本文认为低浓度Cr主要对酿酒酵母细胞质中蛋白造成氧化损伤,浓度升高时则对线粒体DNA也产生氧化损伤。
The present paper is aimed at introducing the author＇ s study of the oxidative damage in yeast Saccharomyces cerevisiae caused by Cadmium. As is known, Cadmium is a kind of toxic heavy metal that can induce oxidative damage. When exposed to CdCl2 dosed 2 μmol/L and 10 μmol/L for 10 hours and when yeast cells were collected for survival assay, oxidative damage of mitochondrial DNA （mDNA） could be found with the level of 8-OH-dG/105dG by HPLE-EC. And when the results were compared between the situations with or without glucose in the medium, it can be concluded that the tolerance of yeast to Cd^2 ＋ would be higher in glucose-free medium than that in glucose-sufficient medium（ P 〈 0.01）. This proves that the yeast in glucose-free medium has had a low level of oxidative damage in mDNA （ P 〈 0.01） at the exposure of 10 μmol/L CdCl2. However, at the exposure of 2 μmol/L CdCl2, there were no signifi- cant differences between the Cd^2＋ tolerance and mDNA oxidative damage （ P 〉 0.05）. Therefore, cadmium with low-dosage can be the main reason for the oxidative damage of cytoplasm protein, however, with the increase of concentration, cadmium would result in the oxidative damage of mDNA.
出处
《安全与环境学报》
CAS
CSCD
2006年第3期77-79,共3页
Journal of Safety and Environment
基金
国家自然科学基金项目(30470302)
关键词
环境毒理
镉
酿酒酵母
氧化损伤
线粒体DNA
葡萄糖
environmental toxicology
cadmium
yeast Saccha romyces cerevisiae
oxidative damage
mitochondrial DNA
glucose
