摘要
目的:探讨晚期糖基化终末产物受体(RAGE)在非胰岛素依赖型糖尿病伴发牙周炎患者牙龈组织中的分布及其在牙周组织破坏中的作用。方法:用免疫组化方法检测9名患者共20个位点牙龈组织中RAGE和肿瘤坏死因子α(TNF-α)的分布,并进行了半定量检测。其中慢性牙周炎(CP)患者5名,非胰岛素依赖型糖尿病(NIDDM)伴发牙周炎患者4名。同时,应用酶联免疫吸附测定法(ELISA)对相应位点龈沟液及外周血中的白细胞介素6(IL-6)及TNF-α进行测量。结果:RAGE阳性细胞在2组的龈组织中的差异具有极显著性,且RAGE的表达与TNF-α的表达显著相关(P<0.05)。2组龈沟液中IL-6及TNF-α浓度的差异无显著性,但二者的浓度在NIDDM组有高于CP组的趋势。结论:RAGE参与了NIDDM伴发牙周炎患者的牙周组织的破坏。
Objective：To detect the distribution of the receptor for advanced glycation end products（RAGE） in gingival tissues of patients with non-insulin dependent diabetes mellitus （ NIDDM ） accompanying periodontitis. Methods：5 patients with chronic periodontitits（CP） and 4 patients with NIDDM accompanying periodontitis were included. Immunohistochemical staining was performed to detect RAGE and tumor necrosis factor α（TNF-α） expression in gingival tissues. Interleukin 6（IL-6） and TNF-αlevels in gingival crevicular fluid and serum of these patients were examined by ELISA. Results：More RAGE cells were observed in the gingival tissus in the patients of NIDDM with CP than in those of CP（ P 〈0.01 ）. The number of RAGE positive cells was significantly correlated with the number of TNF-α positive cells （ P〈0.05 ）. The amount of gingival crevicular fluid, IL-6 and TNF-α levels in gingival crevicular fluid were not significantly different between the two groups （ P〉0.05 ）. Conclusion ： RAGE may play a role in the destruction of periodontal tissues in NIDDM patients with CP.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2006年第3期370-373,共4页
Journal of Practical Stomatology
