摘要
目的探讨卵巢子宫内膜异位症组织中PTEN基因mRNA及蛋白的表达及其临床意义.方法应用半定量逆转录聚合酶链反应（RT-PCR）检测卵巢子宫内膜异位症组织和正常卵巢组织中PTEN基因外显子1-9和5-9的mRNA表达水平,免疫组织化学方法检测其蛋白表达,同时结合病人的临床资料进行分析.结果卵巢子宫内膜异位症组织中PTEN外显子1-9、5-9 mRNA相对表达水平高于正常卵巢组织,差异有显著性（P〈0.01,P〈0.05）.卵巢子宫内膜异位症组织中PTEN mRNA表达水平与病人年龄、囊肿大小无关（P〉0.05）.与临床分期有关（P〈0.05）.卵巢子宫内膜异位症组织中Ⅰ～Ⅱ期有2例细胞核PTEN蛋白呈中等染色表达,与正常卵巢组织中PTEN蛋白的表达比较差异无显著性（P〉0.05）.结论 PTEN mRNA及蛋白表达水平的异常可能是卵巢子宫内膜异位症发生的早期事件.
Objective： To investigate PTEN mRNA and protein expressions in the ovarian endometrioma and its clinical significance. Methods： The expressions of PTEN mRNA and protein were examined by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemistry respectively in the ovarian endometriorna and normal ovarian tissues. The clinical characteristics were compared and clinical significance was analyzed. Results： The mRNA expressions of exons 1-9, 5-9 in PTEN gene in ovarian endometrioma tissues were significantly higher than those in normal ovarian tissues （ P 〈0.01, P 〈0.05）. The expression level of PTEN mRNA in ovarian endometriosis was related to staging （ P 〈0.01 ）, but not to the patient age or the size of cyst （ P 〉0.05）. There was no significant difference in the expression of PTEN protein between ovarian endometrioma and normal ovarian tissues （ P〉0.05）. Conclusion： The abnormal expressions of PTEN mRNA and protein may represent an early event in the genesis of ovarian endometriorna.
出处
《泰山医学院学报》
CAS
2006年第1期26-28,共3页
Journal of Taishan Medical College
