摘要
目的:验证咽拭子法检测EB病毒(EBV)潜伏膜蛋白1(LMP1)在鼻咽癌诊断中应用的可行性;检测LMP1野生型及30bp缺失变异型在鼻咽癌中的分布情况。方法:用咽拭子法收集鼻咽癌组及对照组鼻咽部脱落细胞,微量提取DNA,经PCR扩增人β珠蛋白基因序列验证咽拭子法的可行性,用特异性引物扩增出特异的LMP1序列,验证其在鼻咽癌诊断中的意义。测序分析LMP1变异情况。结果:咽拭子合格率为96.4%,LMP1基因作为鼻咽癌的检测指标,灵敏度为91.7%,特异性为95.6%,其中变异型LMP1在鼻咽癌中的表达频率为80.6%,野生型为11.1%。结论:咽拭子法可作为一种基因检测手段,LMP1基因的检测可以协同EB病毒壳抗原(EBVCA)IgA作为鼻咽癌诊断的辅助指标,在LMP1(+)的鼻咽癌患者中LMP1-30bp缺失突变普遍存在。
Objective：To investigate the feasibility and reliability of detection of Epstein-Barr virus （EBV） latent membrane protein-1 （LMP1） gene by nasopharyngeal swab in the diagnosis of nasopharyngeal carcinoma （NPC）. To investigate the distribution of 30 bp deletion variant of LMP-1 gene in the local population. Method： Nasopharyngeal cells were collected by nasopharyngeal swab, and then DNA was extracted, which was subsequently confirmed by amplification of sequence of β- thalassemia gene by polymerase chain reaction（PCR）. And sequence of LMP1 was amplified with specific primer to verify the significance of LMP1 in the diagnosis of NPC. Result.. DNA was obtained from 96.4% nasopharyngeal swab samples, LMP1 was detected in 33 of 36 samples, while 2 of 45 samples from normal control, with sensitivity 91.7% ,and specialty 95.6%. 30bp deletion of LMP1 gene was found in 80.6 % of NPC samples, and wild type 11.1%. Conclusion：Our study suggests that nasopharyngeal swab could be effective method for gene-detection . As a parameter in diagnosis of NPC, LMP1 gene is maybe superior to EBVCA-IgA. 30bp deletion of LMP1 oncogene is widespread in NPC patients.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2006年第11期499-501,共3页
Journal of Clinical Otorhinolaryngology
关键词
鼻咽肿瘤
潜伏膜蛋白1
咽拭子
Nasopharyngeal neoplasms
Latent membrane protein 1
Nasopharyngeal swab
