摘要
取带１～２个时原基其大小约为０．３７～０．４９ｍｍ的甘薯茎尖，放在ＭＳ＋ＢＡ０．５ｍｇ／Ｌ＋ＮＡＡ０．１ｍｇ／Ｌ＋蔗糖４％＋琼脂６．５ｇ／Ｌ培养基上培养４０ｄ，成苗率为３１．２％～１００％。电镜测出试管苗的脱毒率为９０％。大田试验统计表明、甘薯品种鄂薯１号、“５１－９３”、南薯８８的茎尖脱毒苗比正常薯块苗增产９％～２０．５％。比病苗增产５４．１％～１２８．９％。
The stem tip of sweet potato which was about 0.37-0.49mm long with 1 or 2primordia of leaf had been cultured on a cultural medium of Ms+0.5m/L BA+0.1mg/L NAA+4% sugar+6.5g/L agar for 40 days.Plantlet ratio was 13.2%～100%,and electronic mi-croscope cheek showed that the virus-free ratio of the plantlet amounted to 90%.Statis-tical test of field experiment showed that the yield of virus-free plant of sweet potatoE shu No.1,51-93 and Nanshu No.88 was 9%～20.5% higher than that of their normal plant,and 54.1%128.9% higher than that of the virus plant.
出处
《湖北农业科学》
1996年第2期22-25,共4页
Hubei Agricultural Sciences
关键词
甘薯
茎尖培养
脱毒
sweet potato(Ipomoea batatas),stem tip culture,virus-elimination
