摘要
目的探讨CCK8对RSC364细胞株TNF受体基因表达的影响。方法采用RTPCR法检测不同浓度的八肽胆囊收缩素(CCK8)对在TNF-α诱导下的大鼠滑膜细胞RSC364TNF受体(Tumornecrosisfactorreceptor,TNFR)mRNA表达的影响。结果CCK8在10-6mol/L和10-7mol/L浓度时可抑制TNF-α诱导的TNFR2mRNA在RSC364细胞的表达,且表现了一定的剂量和时间依赖性,CCK受体拮抗剂丙谷胺则可抑制此作用。结论CCK8可抑制TNF-α诱导的大鼠滑膜细胞RSC364株TNFR2基因的表达。
Objective To explore the influence of choleeystokinin oetapeptide （CCK-8） on the expression of TNF receptor （TNFR） mRNA in rat synovial cells （RSC-364）. Methods Detect the expression of TNFR mRNA under induction of TNF-α in the RSC-364 cells containing different concentrations of CCK-8 by RT-PCR. Results The CCK-8 at concentrations of 10^-6 mol/L and 10^-7 mol/L showed dose-and time-dependent inhibitory effect on the expression of TNFP,.2 mRNA in RSC-364 cells under induction of TNF-α,whieh could be reversed by a CCK receptor antagonist proglumide. Conclusion CCK-8 inhibited the expression of TNFR2 mRNA in RSC-364 cells significantly.
出处
《中国生物制品学杂志》
CAS
CSCD
2006年第3期268-271,共4页
Chinese Journal of Biologicals
基金
国家自然科学基金资助项目(30470579).
