摘要
目的：探讨环氧化酶-2特异性抑制剂戊地昔布对人食管癌Eca109细胞凋亡及凋亡基因Fas／FasL表达的影响。方法：采用MTT法检测戊地昔布对人食管癌Eca109细胞生长的抑制作用；流式细胞术（flow cytometry，FCM）检测细胞凋亡和细胞周期分布；电子显微镜检测细胞凋亡形态结构改变；流式细胞术检测凋亡基因Fas和FasL蛋白的表达。结果：戊地昔布（25～400μmol／L）抑制人食管癌Eca109细胞的生长呈时间浓度依赖性；作用72h后，浓度为400μmol／L的戊地昔布对细胞生长的抑制率可达85．95%，凋亡率由（2.38±0．42）％增加到（48．46±0.73）%；50～400μmol／L时增殖指数和S期的细胞比例则明显降低，G0／G1期的细胞比例增加；同时，流式细胞术显示，50～400μmol／L的戊地昔布可上调人食管癌Eca109细胞Fas及FasL蛋白的表达。结论：戊地昔布可诱导人食管癌Eca109细胞发生凋亡，诱导凋亡的机制可能部分与其上调Fas及FasL的表达有关。
Objective：To investigate the effect of COX2 inhibitor, valdecoxib, on the apoptosis and expressions of Fas/FasL in human esophageal cancer cell line Eea109. Methods： MTT method was used to detect the inhibitory effect of valdeeoxib on proliferation of Eea109 cells. Flow eytemetry was used to observe the effect of valdeeoxib on apoptosis and cell cycle distribution of Eea109 cells. Transmission electron microscope was further used to observe the morphological changes of Eea109 cells. The expressions of Fas/FasL were detected by flow eytometry. Results： Valdeeoxib 25 400 mmol/L significantly inhibited the proliferation of Eea109 cells in a time-and dose-dependent fashion. After incubation with valdeeoxib 400 mmol/L for 72 h, the inhibition rate reached 85.95%. The apoptotie ratio increased from（2.38±0.42）% to （48.46±0.73）%. Valdeeoxib50-400 mmol/Lalso decreased the proliferation index and the proportion of cells in the S phase, increased the proportion of cells in the G0/G1 phase, but had no effect on the proportion of cells in the G2/M phase. Valdeeoxib 50 400 mmol/L up-regulated the expressions of Fas and FasL protein in Eea109 cells compared with solvent control. Conclusion： Valdeeoxib induced apoptosis of human Eea109 cells partly by up-regulating the expressions of Fas/FasL.
出处
《肿瘤》
CAS
CSCD
北大核心
2006年第5期440-443,共4页
Tumor
基金
河北省自然科学基金资助项目(编号:301354)
