摘要
对15例髓母细胞瘤手术标本／细胞系内白血病抑制因子（LIF）及其配体基因LIFR和PP130的表达以及LIF对髓母细胞生长的作用进行了研究。结果表明，15例被检样本中，14例有LIF表达和产生，90％以上表达LIFR，IL－6R和gP130中和LIF生物活性可明显抑制细胞生长（P＜0.001）。这提示：内源性LIF对髓母细胞起生长促进作用，阻断LIF生物活性或基因表达可能成为髓母细胞瘤的免疫或基因治疗途径。
In this study, in vivo and in vitro expression of LlF and its signal transducer genes,LIFR and gp130 in medulloblastoma cells and its role(s) in the cell proliferati0n were investigated by dif ferent approaches. The results revealed that 12 out of 13 cases examined were f0und to express LIF andmore than 90% of the samples to express LIFR and gp130. Functional evaluation of LIF using Med-3cells showed that the cell growth and DNA synthesis could be efficiently inhibited (P<0. 001) by neu tralization of LIF bioactivity- Our data thus demonstrated the importance of LIF as autocrtinal growthfactor in the proliferation of medulloblastoma cells and also implicated a potential new therapeutic ap proach for a better treatment of the tumors through blockage of LIF gene expression and/or ligand inter action-.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1996年第1期24-26,共3页
Chinese Journal of Pathology
基金
卫生部优秀青年科技人才基金
辽宁省科委专项基金
