摘要
目的:探讨三氧化二砷(As2O3)对鼠恶性黑素瘤B16细胞诱导凋亡的影响。方法:采用流式细胞术(FCM)检测不同浓度的As2O3诱导B16细胞的早期凋亡率、晚期凋亡率和总凋亡率。结果:As2O3浓度分别为5μmol/L、10μmol/L、20μmol/L时,B16细胞的早期凋亡率分别为7.18%、21.1%、3.59%,晚期凋亡率分别为2.63%、5.35%、6.34%,总凋亡率分别为9.81%、26.45%、9.93%。结论:B16细胞的早期凋亡率和晚期凋亡率对As2O3有浓度依赖关系,高浓度的As2O3使B16细胞出现坏死。
Objective：To study the effect of arsenic trioxide on apoptosis of melanoma B16 ceils. Methods：Early apoptosis rate, late apoptosis rate and total apoptosis rate in melanoma B16 ceils were analysed by flow eytometry. Results：At the doses of arsenic trioxide were 5 μmol/L, 10 μmol/L, or 20 μmol/L, the early apoptosis rates in melanoma B16 cells were 7. 18% ,21. 1%, and 3.59%, respectively;the late apoptosis rates were 2.63% ,5.35%, and 6.34%, respectively;the total apoptosis rates were 9.81% ,26.45%, and 9.93% ,respectively. Coneluslons：The early apoptosis rate and the late apoptosis rate in melanoma B16 ceils have dose-dependent relationship with arsenic trioxide.
出处
《蚌埠医学院学报》
CAS
2006年第3期233-234,共2页
Journal of Bengbu Medical College
基金
安徽省教育厅自然科学研究资助项目(2006kj351b)
