摘要
目的研究苏拉明联合槲皮素对小鼠肺腺癌转移的抑制作用。方法将40只接种高转移性的LA795肺腺癌细胞T739小鼠随机分成5组:对照组、顺铂(DDP)组、槲皮素组、苏拉明组、苏拉明+槲皮素组。实验3周后,处死全部小鼠,取出双肺和剥离皮下肿瘤,计算肺转移发生率,计数各组小鼠肺表面转移结节数及算出肺表面结节转移抑制率,测量各组小鼠肺湿重。免疫组化和图像分析系统检测皮下移植瘤中微血管密度(microvessel density,MVD),血管内皮生长因子(vascular endothelial growth fac-tor,VEGF)的表达并定量分析。结果苏拉明组、槲皮素组与对照组相比肺表面转移结节数明显下降,同时苏拉明组中肺转移发生率、皮下肿瘤内MVD、VEGF的表达与对照组相比也明显下降,相反顺铂组对此则无明显影响。联合用药组则显著抑制肺表面转移结节数且具有协同作用。结论苏拉明和槲皮素对LA795肺腺癌的肺结节转移具有协同抑制作用,苏拉明抑制LA795肺腺癌转移与其抑制其肿瘤内VEGF表达相关。
Objective To study the effect of Suramin combination with Quercetin on lung metastasis of lung aenocarcinoma LA795 in mice. Methods Forty T739 mice bearing high metastasis LA795 cells were randomized into five groups： control group, DDP group, Quercetin group, Suramin group, Quercetin ＋ Suramin group. Different treatments were served from day 4 after transplantation and all mice were sacrificed after 24 days. Lung and Subcutaneous tumors were processed for histological examinatiorL The metastatic tumor foci on lung in mice, lung weight were observed, Lung metastasis occurring rate and metastasis foci inhibitory rate were counted, Subcutaneous tumor microvessel density （MVD）, the related factor of tumor metastasis vascular endothelial growth factor （VEGF） were determined by immunohistochemistry method with image analyses systerm. Results The metastasis foci on lung surface were obviously inhibited by Suramin or Quercetin compared to the control group. Contrary to DDP, the expression of subcutaneous tumor MVD, VEGF and lung metastasis occurring rate were also obviously inhibited by Suramin. The combined group show great synergetic effect in decreasing metastasis foci on lung surface. Conclusion Suramin combination with Quercetin produced additive inhibitory activity against lung metastasis of Lung Adenocarcinoma LA795. The fact that Suramin inhibited lung metastasis of Lung Adenocarcinoma LA795 may be associated its effect by suppressing the expression of VEGF in tumor.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2006年第5期314-316,384,共4页
Cancer Research on Prevention and Treatment
基金
湖南省卫生厅科研基金资助项目(C2005033)
