摘要
目的:探讨苍术炮制前后DNA指纹图谱研究方法。方法:采用RAPD技术对不同产地的茅苍术炮制前后进行DNA指纹图谱研究。结果:不同产地的茅苍术生品DNA指纹图谱一致,几种麸炒苍术的DNA指纹图谱与生品有较大差异。结论:DNA指纹图谱可用于鉴别同种药材的生品。
AIM: To study the DNA fingerprinting of crude and processed Atractylodes lancea. METHODS: DNA fingerprinting of samples of crude and processed Atractylodes lancea were examined by RAPD. RESULTS: All crude Atractylodes lancea showed similar fingerprinting characteristics, while the processed Atractylodes lancea products indicated considerable different results. CONCLUSION: DNA fingerprinting is a reliable method for differentiating crude Atractylodes lancea.
出处
《中成药》
CAS
CSCD
北大核心
2006年第5期674-676,共3页
Chinese Traditional Patent Medicine
基金
国家十五攻关重大课题(编号2001BA701A55-23)
关键词
苍术
RAPD
DNA指纹图谱
Atractylodes lancea
RAPD
DNA fingerprinting of Atractylodes lancea