摘要
脂肪酶是一种广泛应用的水解酶。生物印迹技术是一项新兴技术,它使脂肪酶在有机溶剂中发挥更好的催化能力,如更高的活性和稳定性,从而扩大了脂肪酶作为生物催化剂在工业上的应用。介绍了脂肪酶蛋白质的结构特点,脂肪酶生物印迹的过程和原理,对生物印迹过程中印迹模板的种类和选择原则、保护剂的作用等主要影响因素进行了讨论,并对不同种类脂肪酶的生物印迹条件和适用的反应及生物印迹效果作了归纳总结,指出了目前脂肪酶生物印迹技术存在的问题和发展前景。
Lipase is a kind of widely used hydrolase. Bio-imprinting is a new technology , it induces the lipase to exhibit exciting catalytic ability in nonaqueous solvents, such as higher activity and stability, and enhances lipase＇ s industrial use as bio-catalysts. The characteristic of the lipase＇ s structure, the process and the mechanism of the bio-imprinting were introduced. Influencing factors such as the selective principle of different kinds of bio-imprinting templates and the function of lyoprotectant through the process of the bio-imprinting were discussed. Meanwhile, the condition of the bio-imprinting, the reaction catalyzed by bio-imprinted lipase and the results were concluded. Finally, the problem and the prospect of the bio-imprinting technology were mentioned.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2006年第3期78-82,共5页
China Biotechnology
基金
国家自然科学基金资助项目(20276067)
关键词
生物印迹
脂肪酶
底物
模板
保护剂
Bio-imprinting Lipase Substrate Template Lyoprotectant
