摘要
目的研究亚甲基四氢叶酸还原酶(MTHFR)基因多态性与心肌梗死(MI)的相关性。方法应用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)技术,检测121例心肌梗死患者(患者组)和500名健康人(正常对照组)的MTHFR基因多态性。结果MTHFR基因第677位核苷酸可分为3种类型:CC、CT、TT。患者组3种基因型频率分别为14.0%、46.3%、39.7%,T等位基因频率为62.9%,C等位基因频率为37.2%;正常对照组分别为35.6%、44.0%、20.4%,T等位基因频率为42.4%,C等位基因频率为57.6%。两组间各基因频率及等位基因频率比较差异均具有显著性(P<0.05)。结论MTHFR基因TT基因型,T等位基因与心肌梗死具有相关性。
Objective To investigate the relationship between the polymorphisms of methylenetetrahydrofolate reductase （MTHFR） gene and myocardardial infarction. Methods 121 myocardardial infarction patients and 500 normal controls were recruited in the study, their polymorphisms of MTHFR gene were analyzed by means of polymerase chain reaction and restriction fragment length polymorphism （ PCR - RFLP）. Resuits There were three kinds of genotype： CC（homozygous normal）, CT（heterozygous） and TT （homozygous mutation ）. Among the three genotypes of the patients, CC was accounted for 14.0 %, CT for 46.3 %, TT for 39.7%, while the frequency among control subjects was 35.6 %, 44.0%, 20.4 % ,respectively. The frequency of T alleles was significantly higher in patients than that in normal controls （62.85% and 42.4%, respectively）. There was a significant difference in the frequencies of genotype and alleles between two groups （ P 〈 0.05）. Conclusion There is association among TT genotype, T alleles of MTHFR gene and myocardardial infarction.
出处
《河南职工医学院学报》
2006年第2期87-89,共3页
Journal of Henan Medical College For Staff and Workers
关键词
亚甲基四氢叶酸还原酶
心肌梗死
多态性
methylenetetrahydrofolate reductase
myocardardial infarction
polymorphisms
