摘要
报道了钴对干旱胁迫下大豆幼苗叶片多胺含量、活性氧水平、抗氧化酶活性及细胞膜透性的影响，揭示了钴对干旱胁迫下大豆幼苗叶片的保护作用及机制．结果表明，CoCl2（30μmol·L^-1）可以显著抑制干旱胁迫下乙烯的产生．在干旱胁迫0～12d，CoCl2对叶片丙二醛（MDA）含量和叶绿素含量无显著影响．当干旱胁迫持续15～21d，CoCl2显著抑制叶片多胺氧化酶及二胺氧化酶活性的提高，使腐胺、精胺和亚精胺含量的下降幅度减小．CoCl2显著提高了抗氧化酶的活力，有效抑制了胁迫后期（15～21d）MDA含量的增加，减轻了叶绿索含量和细胞膜稳定指数的下降．可以认为，当大豆幼苗叶片由于持续干旱而遭受较为严重的细胞伤害时，CoCl2通过抑制乙烯产生、抑制多胺含量下降以及提高抗氧化酶的活性，缓解了活性氧的累积和细胞膜透性的破坏，对干旱胁迫下大豆幼苗叶片具有一定的保护作用．
This paper studied the effects of cobalt on the polyamine content, reactive oxygen level, anti-oxidative enzyme activity, and cell membrane permeability of soybean seedling＇s leaf under drought stress, and discussed the mechanisms of these effects. The results showed that under drought stress, cobalt at its concentration of 30 μmol·L^-1 could significantly inhibit the production of ethylene. This concentration of cobalt had no significant effects on the contents of MDA and chlorophyll when the drought stress lasted 0～12 days, but significantly restrained the increase of reactive oxygen level, hampered the decline of putrescine, spermidine and spcrmine contents and anti-oxidative enzyme activities, and thus, inhibited the accumulation of MDA and alleviated the reduction of chlorophyll content when the stress lasted 15～21 days. It could be concluded that when soybean seedling＇s leaf was deeply stressed and damaged under drought stress, cobalt could alleviate the decline of polyamine content and anti-oxidative enzyme activity, and hence, alleviate the increment of reactive oxygen level and cell membrane＇s damage degree through inhibiting the production of ethylene, and exerted its protective effects on the soybean seedling＇s leaf under drought stress.
出处
《应用生态学报》
CAS
CSCD
北大核心
2006年第5期796-800,共5页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(30170547)
国家博士后科学基金资助项目(20040350711)
关键词
干旱胁迫
氯化钻
大豆
乙烯
多胺
活性氧
Drought stress, Cobalt chloride, Soybean, Ethylene, Polyamine, Reactive oxygen.
