摘要
目的:探讨卡托普利体外对人精子运动参数的影响。方法:将卡托普利注射液配制成1×10-5、1×10-6及1×10-7mol/L 3组浓度,分别在体外作用于取自正常生育男性并经Percoll梯度离心处理的精子。分别将3组卡托普利溶液各20μl与180μl精子悬液混匀,均于作用5 m in后采用计算机辅助精液分析系统检测精子运动参数。结果:3组不同浓度卡托普利溶液与精子作用5 m in后,精子活率和前向运动精子百分率明显降低(P<0.05);其他运动参数无明显变化。结论:卡托普利能显著降低精子活率和前向运动精子百分率,对其他运动参数无显著影响。
Obejctive： To determine the effects of captopril on human sperm motility in vitro. Methods ： Sperm specimens were aspetically obtained by masturbation and prepared by Percoll gradient-centrifugation technique to produce a spermatozoa suspension of high motility from 12 healthy fertile men. The seprmatozoa suspension were incubated with captopril at 1：9 ratio of volume at 37 ℃ in vitro, which was administrated at three different concentrations. Measurements were carried out within 5 minutes for all specimens including VAP, VSL, VCL, STR, ALH and progressive motility. Results ： Within 5 minutes, three different concentrations of captopril could change the human spermtozoa motility and forward motile sperm percentage, but captopril did not decrease the other motility parameters, including VSL, VCL and VAP etc. The high concentration group could decrease the VCL more than those of the other two groups and it seemed that the velocity parameters of the middle concentration group were higher than those of the other two groups, but there were no significant difference statistically. Conclusion： It suggests that captopril can decrease the human spermatozoa motility and forward motile sperm percentage, but it can not directly affect the spermatozoa velocity in vitro.
出处
《中华男科学杂志》
CAS
CSCD
2006年第5期435-437,共3页
National Journal of Andrology
