摘要
目的:探讨人精液中性激素水平与生殖细胞凋亡的关系。方法:随机选择66例不育患者及30例生育男性,采用ELISA法进行卵泡刺激素(FSH)、黄体生成素(LH)、泌乳素(PRL)和睾酮(T)水平分析,并用脱氧核苷酸末端转移酶(TdT)介导的缺口末端标记(TUNEL)法检测生殖细胞的凋亡。结果:精液中FSH、LH、PRL和T水平30例生育者分别为(1.63±0.15)U/L、(2.18±0.21)U/L、(6.34±0.30)nmol/L、(1.85±0.11)nmol/L,生殖细胞凋亡率为(4.61±1.23)%,而66例不育者4种性激素水平分别为(1.25±0.18)U/L、(1.76±0.32)U/L、(5.86±0.31)nmol/L、(1.45±0.13)nmol/L,生殖细胞凋亡率为(18.36±2.04)%。两者相比各参数差异均有显著性(P<0.01)。不育组FSH、LH、PRL、T水平与生殖细胞的凋亡率呈负相关(r分别为-0.88、-0.93、-0.90、-0.98,P均<0.01)。凋亡的生殖细胞体积缩小,核染色质致密,凝聚在核周形成新月形,或核裂解形成凋亡小体。结论:精液性激素水平与生殖细胞的凋亡有着密切关系,低水平的性激素可能导致睾丸生殖细胞凋亡率增加。
Objective： To explore the relationship between sexual hormones in semen and germ cell apoptosis in male population. Methods： Sixty-six infertile patients and thirty fertile males were selected randomly. The levels of folicle stimulating hormone（ FSH）, prolactin （PRL）, luteinizing hormone （LH）, and testosterone （T） in semen were measured by ELISA. Terminal deoxynucleotidyl transferase mediated UTP nick end labeling （TUNEL） was used for the detection of germ cell apoptosis. Results： The levels of FSH, LH, PRL, T in thirty fertile men were （ 1.63 ± 0.15 ） U/L, （ 2.18 ± 0.21 ） U/L, （ 6.34 ± 0.30 ） nmol/L, （ 1.85 ±0. 11 ） nmol/L, respectively, and germ cell apoptosis rate was （4.61±1.23）%. FSH, LH, PRL, T levels in infertile group were （1.25±0. 18） U/L, （ 1.76 ±0.32） U/L, （ 5.86 ±0.13 ） nmol/l, （ 1.45 ± 0.13 ） nmol/l, respectively, and germ cell apoptosis rate was （ 18.36 ± 2.04 ） %. There were significant differences in all parameters between infertile group and fertile group. The levels of FSH, LH, PRL,T were negatively correlated with germ cell apoptosis rates（r=-0.88,-0.93,-0.90,-0.98）.The volume of apoptotic germ cell decreased,and chromatin was compacted to form cell-membrane blebs and apoptotic bodies. Conclusion：Low concentration of sexual hormones may increase the apoptosis of germ cells,which can induce maleinfertility.
出处
《中华男科学杂志》
CAS
CSCD
2006年第5期432-434,共3页
National Journal of Andrology
基金
第四军医大学西京创新基金资助(04045L11)
关键词
性激素
生殖细胞
凋亡
精液
sexual hormone
germ cell
apoptosis
semen
