摘要
目的检测端粒酶hTERT即端粒酶逆转录酶在人BGC-823胃腺癌细胞中的表达,探讨其临床应用的价值。方法应用ELISA(酶联免疫吸附法)检测胃腺癌细胞裂解液中端粒酶hTERT的活性表达。结果当检测胃腺癌的细胞数为5×106/50μL时为可疑阳性,当细胞数为1×107/50μL时为阳性,而当检测的细胞数为2.5×106/50μL和1.25×106/50μL时均为阴性。结论通过检测端粒酶hTERT可以说明端粒酶作为肿瘤的一种标志物存在与胃腺癌细胞中,而且应用ELISA法检测端粒酶的活性既准确又简便。
Objective To detect expression of the hTERT （ human telomerase rcverse transcriptase） activity in human BGC- 823 gastric adenoma cells and to study the hTERT practical value in the clinic. Methods Using the ELISA （the enzyme-linked immunosorbent ashy） examined the activity of human telomerase reverse transcriptase in the splitting liquid of the gastric adenoma cells. Results The number of the examing cells being 5×10^6/50μL, the result was impossible telomerase -positive. The number being 1× 10^7/50μL was telomerase- positive. The number being 2.5 × 10^6 / 50μL and 1.25 ×10^6/50μL were all telomerase - negative. Conclusion Though detecting the activity of telomerase hTERT, explained that the telomerase is a kind of tumor marker in the human gastric adenoma cells. Using the ELISA method can accurate and convenient detect the activity of the telomerase.
出处
《锦州医学院学报》
2006年第1期15-17,共3页
Journal of Jinzhou Medical College
