摘要
在比较组织块法和消化法分离培养猪前体脂肪细胞的基础上,通过对前体脂肪细胞增殖与分化过程中细胞的形态学变化、生长曲线、油红O染色以及脂肪细胞特异性标志基因脂蛋白脂酶(lipoproteinlipase,LPL)和过氧化物体增殖剂活化受体γ(peroxisomeproliferators-activatedreceptorγ,PPARγ)表达的研究,证明用消化法可从猪的脂肪组织中分离获得大量的前体脂肪细胞,其生长旺盛,可见自发充脂;传代细胞经诱导培养后,充脂率大幅度提高,脂肪特异性标志基因表达增强。这为深入研究脂肪细胞增殖与分化以及猪体脂肪沉积提供了一个较好的模型。
Explant techniques and monolayer culture in culturing porcine preadipocytes were compared, Cells obtained from monolayer culture are more than from explant techniques in a short period of time, but we have to purify cells by successive passage culture. Subcultured cells were plated in differentiateon medium, analyzed by their morphological changes, growth curve, oil red O staining and lipoprotein lipase gene, peroxisome proliferatorsactivated receptor γ gene expression. Results shown a lot of preadipocytes could be harvested from adipose tissue samples obtained from crossbreed piglets, these preadipocytes were typical fibroblasts with normal morphology, proliferative and had some extent differentiation rate in primary culture. Under controlled condition, especially purified by subclutre, these preadipocytes replayed their hyperplasia process with higher accumulation of drop lipid .This study laid the basis for further probing into adipocyte proliferative, differentiation and fat deposition.
出处
《细胞生物学杂志》
CSCD
2005年第6期693-696,共4页
Chinese Journal of Cell Biology
基金
国家重点基础研究发展规划(973计划)项目资助(No.2004CB117506)~~
关键词
猪
前体脂肪细胞
传代
诱导
porcine
preadipocyte
subculture
differentiation
