摘要
目的建立救心丸中华蟾酥毒基和脂蟾毒配基的高效液相色谱含量测定方法。方法WatersSymmetryShieldRP18(3.9mm×250mm,5μm),流动相为乙腈-0.5mol/L磷酸二氢钾溶液(用磷酸调节pH值至3.2)(42∶58);柱温30℃;流速1.0mL/min;检测波长为296nm。结果华蟾酥毒基线性范围为0.0696～0.8352μg,精密度试验RSD为0.53%,稳定性试验RSD为0.71%,重现性试验RSD为4.83%,回收率为101.86%,RSD为1.81%;脂蟾毒配基线性范围为0.1576～0.9456μg,精密度试验RSD为0.15%,稳定性试验RSD为0.76%,重现性试验RSD为5.00%,回收率为102.07%,RSD为1.62%。结论本方法快速、简便、准确、重现性较好,结果可靠,可为救心丸的质量评价提供有效手段。
Objective To estalish an accurate method for the determination of cinobufagin and resibufogenin in Jiuxin Tablet. Method Waters Symmetry Shield RP18 （3.9 mm×250 mm,5 μm） column in an oven at 30℃ was need, with a mobile phase consisting of acetonitrile-0.5mol/L kalium dihydrogen phospate （phosphoric acid preparating pH=3.2） （42 ： 58） and a UV detector at 296mm, the flow rate was 1.0 mL/min. Results The linear ranges of Cinobufagin was 0.0696～0.8352μg. The RSD of measurement precision test was 0.53%, the average recovery was 101.86% （RSD=1.81%, n=5）, the linear ranges of Resibufogenin was 0.1576～0.9456 μg, the RSD of measurement precision test was 0.15%, the average recovery was 102.07% （RSD=1.62%, n=5）.Conclusion The method was simple and accurate, and can be used for the quality control of Jiuxin tablet.
出处
《中国中医药信息杂志》
CAS
CSCD
2006年第5期44-45,共2页
Chinese Journal of Information on Traditional Chinese Medicine
