摘要
从斜带石斑鱼(Epinephelus coioides)白细胞cDNA文库随机挑选克隆,测定表达序列标签(ESTs),利用BLASTX程序与GenBank中的序列进行比较,得到了1个cDNA,其编码蛋白质与人类DNA聚合酶δ(polδ)的P12亚基有47.7%的同源性,推测它为斜带石斑鱼polδ的12 kD小亚基的cDNA,所编码的蛋白质命名为P12。该cDNA全长619 bp,含1个330 bp的开放阅读框,编码109个氨基酸残基的蛋白质。序列对比表明,斜带石斑鱼的氨基酸序列与人、大鼠、小鼠、拟南芥和裂殖酵母的polδ小亚基有同源性,同时与斑马鱼EST CK680540的编码序列有66.6%的同源性。用RT-PCR检测该基因在石斑鱼各组织的表达情况,发现在腮、肝、脾、全血、胃和后肾有表达,而在肠、心脏、脑和头肾未检测到。
Orange-spotted grouper （Epinephelus coioids ） leukocyte cDNA library was constructed and the expressed sequence tags （ESTs） were selected and sequenced. The ESTs were compared with those in GenBank Database using BLASTX program. One of the sequences showed 47.7 % identity to human polδ P12 subunit and was regarded as polδ P12 subunit of orange-spotted grouper, The putative encoding protein was designated P12. The isolated cDNA from orange-spotted grouper was 619 bp in length. The longest open reading frame was 330 bp in length and encoded a protein of 109 amino acid residues. The sequence alignments among polδ P12 of orange-spotted grouper and human, mouse, rat, Arabidopsis thaliana and budding yeast showed significant similarity. With RT-PCR, the polδ P12 subunit mRNA transcripts were detected in gill, liver, spleen, blood cells, stomach, posterior kidney of E. coioides, but not in the intestine, heart, brain and interior kidney. It is the first report of the polδ P12 subunit in fish.
出处
《中国水产科学》
CAS
CSCD
北大核心
2006年第3期466-470,共5页
Journal of Fishery Sciences of China
基金
国家自然科学基金资助项目(30300266)
国家"863"高技术研究发展计划项目(2001AA601010)
广东省科技计划重点引导项目(2004B20301008)
