摘要
本文首次报道了鹅源鸭瘟病毒(DPV—Ⅰ)和鸭胚化小鹅瘟病毒(GPV—Ⅰ)能同时在同一鸭胚内复制增殖,未发现干扰作用,在理论上说明某些不相关的两种病毒可在同一宿主增殖,实践上为利用同一鸭胚研制二联疫苗提供了依据。研究结果表明:1.DPV—Ⅰ和GPV—Ⅰ联合感染同一鸭胚后,其尿囊液在电镜下见两种病毒,DPV—Ⅰ呈园形或椭园形,有囊膜,直径为38—109nm,GPV-Ⅰ呈园形,无囊膜,直径为18—25nm;2.含毒尿囊液使鸭胚成纤维细胞(DEF)单层发生细胞病变作用(CPE),证实存在DPV-Ⅰ,而用小鹅瘟微量免疫扩散(MID)试验,又能检出GPV-Ⅰ抗原;3.含毒尿囊液免疫鹅的血清中存在抗两种病毒的(?)和抗体和GPV沉淀抗体;4.含毒尿囊液免疫的成鹅对DPV强毒攻击有相当免疫力,免疫鹅血清能中和GPV,使其失去对鸭胚的致病力;5.GPV-Ⅰ单独或与DPV-Ⅰ联合感染DEF单层后,均未见在细胞上复制。
It is the first time in the present paper to report the propagation of duck plague virus (DPV-Ⅰ) and gosling plagae virus (GPV-Ⅰ) in the identical duck embryo, what have been revealed in the results are as follows: 1. Both DPV and GPV particles were detected under electronic microscope in the CAF of the duck embryoes infected with DPV-Ⅰ and GPV-Ⅰ. 2. CAF with viruses could produce eytopathic effect (CPE)in the duck embryonic blast (DEF)cell monolayers, showing the existence of DPV; and GPV antigens were detected by micro-immune cliffusion (MID)test for gosling plague. 3. The neutralization antibodies to both DPV/GPV and GPV-precipiting antibody were detected in the adult geese vaccinated with DPV/GPV, and 4. The vaccinated adult geese were resistant to the challenge with virulent DPV 16 days after vaccination. It has also been found that GPV-Ⅰ could not be propagated solely or coinfected with DPV-Ⅰ in the DEF cells.
出处
《病毒学杂志》
CAS
CSCD
1990年第1期102-106,共5页
