摘要
目的研究银杏叶提取物(Extracts of ginkgo b iloba leaves,EGB)对β淀粉样蛋白(Aβ25-35)诱导的PC12细胞损伤的保护作用。方法用Aβ25-35处理诱导体外培养的PC12细胞损伤,并用EGB进行保护,显微观察细胞形态的改变,LDH法和MTT法检测细胞膜的损伤情况。结果EGB处理组细胞数比损伤组显著增多,受损变圆的细胞数较少,细胞形态明显改善,EGB处理组上清液LDH活性显著低于Aβ25-35处理组(P<0.01),MTT值显著高于Aβ25-35处理组(P<0.01)。结论EGB对Aβ25-35诱导的PC12细胞损伤具有显著的保护作用。
Objective To study the protective effects of extracts of Ginkgo biloba leaves on the damaged PC12 cells induced by amyloid 13 peptide （Aβ25-35）. Methods PC12 cells were treated with Aβ25-35 to induce injury in vitro, extracts of Ginkgo biloba leaves was added into the culture to test its protective effect. The morphology of PC12 cells was observed by microscope. The cellular membrane injury was measured by LDH and MTF assays. Results The number of cells was higher and the number of the damaged cells was lower in protective group than those in damaged group. LDH activity was lower obviously （ P 〈 0.01 ） and MTr value was higher significantly in protective group （P 〈 0.01 ） than those of damaged group. Conclusions Extracts of Ginkgo biloba leaves has markedly protective effect on the damaged PC12 cells induced by Aβ25-35.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2006年第4期488-490,共3页
Chinese Journal of Gerontology
基金
广东省科技厅立项课题(粤科社字〔2004〕139号)
