摘要
目的探讨二甲双胍联合未成熟卵体外培养成熟(IVM)对氯米芬抵抗的多囊卵巢综合征(PCOS)的治疗效果。方法2003-10-2005-03将安徽医科大学第一附属医院56例氯米芬抵抗的PCOS患者随机分成两组,二甲双胍组(28例)给予二甲双胍500mg,每日2次,12周后进行34个IVM周期治疗;对照组(28例)直接行36个IVM周期治疗。比较两组获卵数、成熟率、受精率、优质胚胎形成率和妊娠率。荧光原位杂交(FISH)方法检测两组体外培养成熟的卵母细胞(MⅡ)13、16、18号染色体非整倍体情况。结果二甲双胍组和对照组每周期获未成熟卵数(12·35±6·29)个、(9·39±6·17)个,成熟率76·67%、70·41%,受精率69·57%、67·65%,卵裂率87·50%、89·44%,经比较差异均无显著性意义(P>0·05);两组优质胚胎形成率37·76%、24·31%,妊娠率38·24%、16·67%,经比较差异有显著性意义(P<0·05)。两组经体外培养成熟的卵细胞染色体非整倍体形成率差异无显著性意义(P>0·05)。结论对氯米芬抵抗的PCOS患者予以二甲双胍预治疗,可改善IVM的治疗效果,提高临床妊娠率。
Objective To evaluate the effects of mefformin adminstration on in vitro maturation in a group of polycystic ovary syndrome patients with clomiphene citrate resistance. Methods From Oct. 2003 to Mar. 2005,56 PCOS women with clomiphene citrate resistant in the First Affiliated Hospital of Anhui Medical University were randomly divided into two groups. The study group （28 cases） took 500mg mefformin twice a day for 12 weeks and then received 34 cycles of IVM program. The controls directly received 36 cycles of IVM program. The number of immatured oocytes retrieved and the rates of oocyte maturation, fertilization, good quality embryo and pregnancy were compared between the two groups. The incidence of chromosomes aneuploidy of maturation oocytes in vitro in the two groups were detected by Fluorescence In Situ Hybridisation. Results There were no differences in the number of immatured oocytes retrieved （ 12.35 ± 6.29 vs 9.39 ± 6.17） and the rates of oocyte maturation （ 76.67 % vs. 70.41% ）, fertilization and cleavage rates （ 69.57 % vs. 67.65% and 87.50% vs. 89.44%, respectively）between the two groups. The good embryo quality rates and pregnant rates （37.76% vs. 24.31% and 38.24% vs. 16.67%, respectively） in the mefformin group were significantly higher than those of control group （ P 〈 0.05 ）. The incidence of chromosomes aneuploidy of maturation oocytes in the two groups were similar（P 〉 0.05）. Conclusion Pre-treatment with mefformin may improve curative effect of IVM on the PCOS patients with clomiphene citrate resistance and the clinical pregnant rates.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2006年第5期345-348,共4页
Chinese Journal of Practical Gynecology and Obstetrics
基金
安徽省教育厅自然科学基金资助项目(2003kj213)
