摘要
AIM： To study the SSTR1, 2, 3, 4, 5 expression and their relationships with clinico-pathological factors, cell proliferation, Bcl-2 and p53 expression in colorectal cancer cells. METHODS： Immunohistochemical staining of five SSTR subtypes, Ki-67, Bcl-2 and p53 was performed by the standard streptavidin-peroxidase （SP） technique for the paraffin sections of 127 colorectal cancers, and expression of five SSTR subtypes in 40 specimens of normal colorectal mucosae was detected with the same method. RESULTS： Positive staining for five SSTR subtypes was observed in colorectal cancer cells and normal colorectal mucosae. SSTR1 was the most predominant subtype in both colorectal cancer and normal colorectal mucosa, and the second was SSTR5 or SSTR2. As compared with normal colorectal mucosa, SSTR4 was more frequently expressed in colorectal cancer cells （2.5% vs 18.9%, P〈 0.05）; the expression of SSTR2, 4, 5 in moderately to well differentiated colorectal adenocarcinoma was significantly higher than that in poorly differentiated ones （P〈 0.05）, the SSTR1 expression in colorectal cancer with positive lymph node metastasis was significantly higher than that with negative lymph node metastasis （72.2% and 54.5%, P〈 0.05）. In addition, in the ulcerative type of colorectal cancer, SSTR2 expression was obviously decreased （P 〈 0.05）; the correlation did not reach a statistical significance between the five SSTR subtypes expression and Dukes＇stages （P〉 0.05）, but the frequency of SSTR1 expression increased with Dukes＇ stage, while SSTR3 and SSTR5 expression decreased with Dukes＇ stage. Moreover, there was no correlation between expression of the five SSTR subtypes and other clinicopathological factors such as age, sex, tumor site, tumor depth, distant metastasis. The proliferative indexes in colorectal cancer cells with negative expression of SSTR2 and SSTR3 were significantly higher than that with positive expression （P〈0.05）. The Bcl-2 expression in colorectal cancer cells wit
瞄准：学习 SSTR1， 2， 3， 4，有在颜色的 clinico 病理学的因素，房间增长， Bcl-2 和 p53 表示的 5 表示和他们的关系表面的癌症房间。方法：五种 SSTR 子类型， Ki-67， Bcl-2 和 p53 染色的 Immunohistochemical 被标准 streptavidin-peroxidase (SP ) 执行为 127 颜色的石蜡节的技术表面的癌症。并且在正常的 40 个标本的五种 SSTR 子类型的表示渲染表面的 mucosae 与一样的方法被检测。结果：为五种 SSTR 子类型的积极染色在颜色被观察表面的癌症房间和正常颜色表面的 mucosae。SSTR1 是最占优势的子类型在渲染表面的癌症，正常渲染表面的粘膜，并且第二是 SSTR5 或 SSTR2。作为与正常相比渲染表面的粘膜， SSTR4 更经常在颜色被表示表面的癌症房间(2.5% 对 18.9% ， P<0.05 ) ； SSTR2 的表示， 4 ， 5 在里面中等到很好区分的颜色，表面的腺癌在糟糕区分的( P<0.05 )比那显著地高，在有积极淋巴节点转移的表面的癌症是的颜色的 SSTR1 表示比那显著地高与否定淋巴节点转移(72.2%和54.5%， P<0.05 )。另外，颜色在溃疡的打表面的癌症， SSTR2 表示显然被减少(P<0.05 ) ；关联没到达在五 SSTR 子类型表情和 Dukes'stages (P>0.05 ) 之间的统计意义，但是 SSTR1 表示的频率与 Dukes'stage 增加，当 SSTR3 和 SSTR5 表示与公爵的阶段减少了时。而且，在象年龄那样的五种 SSTR 子类型和另外的 clinicopathological 因素的表示之间没有关联，性别，肿瘤地点，肿瘤深度，远转移。在肤色的增生的索引有 SSTR2 和 SSTR3 的否定表示的表面的癌症房间与积极表示(P<0.05 ) 比那显著地高。在肤色的 Bcl-2 表示有 SSTR1 的积极表示的表面的癌症房间， 2， 3， 5 与否定表示(P<0.05 ) 是比那显著地低的。在五种 SSTR 子类型和 p53 表示之间没有关联。结论：最占优势的 SSTR 子类型是 SSTR1，并且第二是 SSTR2 或 SSTR5。五�
基金
Supported by Youth Scientific Research Foundation of Health Department of Fujian Province. No.2003-1-11
