摘要
转座子在脊椎动物中的应用远落后于在其他生物系统中的应用。“睡美人”转座子(sleeping beauty transposon)是Tc1/mariner转座因子超家族中的一员,是存在于鲑鱼基因组中的1个已经失活的转座子。1997年,Ivics等将这个转座子进行重建并恢复了它的活性。短短几年内的有关研究表明,“睡美人”转座子是目前在脊椎动物中转座活性最高的转座子。结合该转座子系统逐步显示出的广阔应用前景,本文重点论述了其结构、转座机制及应用,并提出了应用“睡美人”转座子系统须注意的问题。
The application of transposon in vertebrate is far behind of the use in other biological systems. Sleeping beauty （SB） transposon is a Tcl/mariner-like transposon that was reconstructed from the salmonid genome by restoring its activity through the correction of accumulated mutations in 1997. The study during the few years showed that SB transposon was the transposition in vertebrates with the highest transposition activity. This paper emphasizes the structure, the transposition mechanism and the application of SB transposon. Several issues of the application of SB transposon were also mentioned.
出处
《生物技术通讯》
CAS
2006年第2期224-227,共4页
Letters in Biotechnology
基金
辽宁省科技基金项目(9810100302)
关键词
“睡美人”转座子
转座
转基因
sleeping beauty transposon
transposition
transgenic
