摘要
目的:研究红枫组织培养中增殖阶段的培养基,以期筛选出最适增殖培养基配方,为最终建立红枫无毒快繁体系奠定基础。方法:以MS培养基为基本培养基,附加不同浓度的CPPU和6-BA,并以食用白砂糖代替分析纯蔗糖,对红枫进行增殖培养,比较生长状况。结果:CPPU和6-BA均能促进红枫单芽茎段的腋芽增殖,其中以CPPU的效果较好。结论:最适增殖培养基为(MS,30g/L蔗糖,6.5g/L琼脂,CPPU0.3mg/L,IBA0.05mg/L),可以用食用白砂糖代替分析纯蔗糖对腋芽进行增殖培养。
Objective： The culture medium of multiplication of red maple was studied in the experiment. The purpose was to select the optimal medium and to found the viral-free rapid propagation system finally. Methods： CPPU and 6-BA were supplemented in MS basal medium, and sucrose of analytical pure was replaced by eatable sugar. Results： The study showed that CPPU and 6-BA could promote multiplication of the axillary bud on stem segment with single bud. And CPPU was better than 6-BA for multiplication. Conclusion： a formula for optimal medium consisted of MS, 30 g/L sucrose, 6.5 g/L agar, CPPU 0.3 mg/L, IBA 0.05 mg/U In addition, sucrose of analytically pure could be replaced by eatable sugar in the medium.
出处
《生物技术通讯》
CAS
2006年第2期198-200,共3页
Letters in Biotechnology
基金
河北省建设科学技术研究项目(2004-110)
关键词
红枫
增殖培养
培养基
red maple
multiplication culture
medium
