摘要
目的观察抑制转化生长因子(TGF)β1表达对大肠癌细胞生长周期和侵袭力的影响。方法用小片段TGβ1的双链RNA(siRNA)转染大肠癌HCT116细胞,半定量RT-PCR法检测 TGFβ1受抑程度,流式细胞仪观察转染后细胞周期变化,软琼脂克隆实验检测肿瘤细胞生长及成瘤性的变化。结果 TGFβ1的小片段RNA转染显著抑制了肿瘤细胞TGFβ1的表达,使细胞HCT116的S 期细胞较对照组明显增高59．0％-82．5％,进入G2期细胞减少;转染组细胞克隆形成率较对照组显著下降。结论 RNA干扰技术可快捷方便地阻断TGFβ1表达,使大肠癌细胞S期阻滞并抑制肿瘤细胞生长、降低其成瘤性。
Objectives To observe the effect of inhibition of transforming growth factor （TGF）β1 on the growth and aggression of colon cancer cells by RNA interference. Methods HCT116 colon cancer cells were transfected with small interfering dsRNA （siRNA）, the effect of the inhibition of TGFβ1 was tested by semi-quantitative RT-PCR, and changes in cell cycle distribution were analyzed by flow cytometry, the ability of aggression was detected by soft agar colony assay. Results The inhibition of the expression of TGFβ1 was significantly detected after the transfection of siRNA against TGFβ1, which induced the increase of S phase by 59.0%-82. 5% and decrease in G2 phase, colony assay further demonstrated that siRNA against TGFβ1 led to a significant reduction in colony formation as compared with the control group. Conclusions The present data suggested that the expression of TGFβ1 was conveniently and rapidly blocked by siRNA transfection, and this may lead to the cell cycle arrest and inhibition of the growth and tumorigenesis of colon cells.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2006年第3期167-170,共4页
Chinese Journal of Digestion
基金
卫生部内科消化重点实验室资助
