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酶法与抗体阻碍均相法测定高密度脂蛋白胆固醇试剂比较

The Compaison of Enzymic Method and Antibsdy Hindrance Homogenous Method to Determine the High-density Lipoprotein Cholesterol
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摘要 目的对两种方法检测血清中的高密度脂蛋白进行应用评价.方法运用抗体阻碍均相法与酶法(直接法)做精密度、准确度、线性、对比试验观察.结果精密度测定结果抗体阻碍均相法批内CV为0.92%~3.17%,批间CV为1.27%~3.43%,直接法批内CV为1.41%~3.35%,批间CV为1.65%~3.87%.回收试验96.4%~103%;抗体阻碍均相法性试验结果相关系数0.9996;直接法线性试验结果相关系数为0.9992.阻碍均相法与直接法对比测定结果相关系数为0.9845.结论国产试剂(直接法)与进口(抗体阻碍均相法)试剂相比有较好的重复性、准确性、线性及相关性,并可在多功能生化分析仪上使用.而进口试剂价格昂贵,因此国产试剂的使用完全满足临床需要. Objective To evaluate two kinds of methods of detecting the high-density lipoprotein cholesterol(HDL- C)in serum. Methods Antihady hindrance homogenous method and enzyme method(direct method)were used in preision experiment accuracy experiment, linear experiment and central experiment. Result The results of preision detecting are fouowing: The CV of antibady hindrance homogenous method in groups is 0.92 % - 3.17 % and between groups is 1.27 % - 3.43 % On the other hand,the CV of direct method in groups is 1.41 % -3.35% and between groups is 1.65% -3.87%. Inrecycling experiment, the CV is 96.4% -103 %. The correlative factor of linar experiment by Antibady hindrance homogenous method is 0. 9996, and with direct method, it is 0. 9992. The correlative factor in the result that generates from the comparison between antibady hindrance homogenous method and direct method is 0. 09845. Conclusion Compared with import reagent (antibady hindrance homogenousmethod), the domestic reagent (direct method) really exists better repetitveness, accuracy, linear and dependency, and can fully supply the clinical need.
出处 《实用全科医学》 2006年第3期345-345,347,共2页 Applied Journal Of General Practice
关键词 高密度脂蛋白 抗体阻碍均相法 酶法 High-density lipoprotein cholesterol Antibady hindrance homogenous method Enzyme method
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