摘要
目的分析我国疟疾混合流行区云南分离株间日疟原虫(P.v)传播阻断疫苗候选抗原Pvs28基因特点。方法收集云南省血样17份;提取疟原虫基因组DNA;PCR扩增Pvs28基因;基因测序;DnaSPversion4.0软件进行基因多态性分析。结果成功扩增Pvs28全长基因17个序列。与标准株Sal-I比较,检测出7个错义突变,7个基因型和7种氨基酸型。云南P.v分离株核苷酸多态性π值为0.0044。若不考虑重复片段拷贝数的差异,云南P.v分离株和湖北P.v分离株Pvs28蛋白主导氨基酸型完全一致,即V14-L52-L98-E105-L116-S140-I223。与泰国P.v分离株比较,我国主导基因型突变位点完全包含在泰国P.v分离株突变位点中。结论以Sal-IPvs28为基础建立的传播阻断疫苗能够克服云南P.v分离株Pvs28抗原多样性而发挥传播阻断作用,提示间日疟原虫传播阻断疫苗在我国具有良好的应用前景。
The genetic polymorphism of the candidate antigen Pvs 28 for the transmission-blocking vaccine of vivax malaria from Yunnan isolates was analyzed. Seventeen blood samples were collected in the epidemic area of malaria in Yunnan province, and the genomic DNA of malaria parasites was extracted from the collected samples. The Pvs 28 gene was amplified by PCR, and the PCR products were directly sequenced. The genetic polymorphism was analyzed by DnaSP version 4.0 software. In the present study, the full length of Pvs 28 gene with 17 segments of sequences was successfully amplified. Compared with Sal-I sequence, 7 non-synonymous mutations sites, 7 varieties of haplotype and 7 amino acid substitutions were detected. The nueleotide diversity showed that the n value was 0. 0044. The predominant amino acid type was V^14-L^52-L^98-E^105-L^115-S^14-I^122 Regardless of the differences in repeat region of Pvs 28, the predominant amino acid type of Yunnan isolates and Hubei isolates was the same. Compared with the sequences from Thailand isolates, the mutation sites of main haplotyes were included in those of Thailand isolates, It suggests that the vivax transmission-blocking vaccine based on Pvs 28 could overcome the limited genetic mutation and would promise a good prospect for a wide use in China,
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2006年第4期360-362,293,共4页
Chinese Journal of Zoonoses
基金
国家自然科学基金资助项目(No.30371347)
关键词
间日疟原虫
传播阻断疫苗候选抗原
PVS
28
基因多态性
Plasmodium vivax
good prospect for a wide use in China, TBV antigen
Pvs 28
genetic polymorphism
