摘要
通过设计合适的引物,经RT-PCR方法从里氏木霉的总RNA中扩增出Swollenin基因,并将该基因连接到PMD18-T Simple载体,得到该基因的克隆菌株,经酶切并将该基因连接到巴氏毕赤酵母表达载体pPICZαA,得到重组质粒pPICZαA-swo1.将该重组质粒线性化后,通过电转化方法转化毕赤酵母GS115,得到重组菌株P.pastoris-swo1.该菌株的发酵液可使滤纸纤维强度降低,纤维素膨胀及崩解效果明显,表明重构表达的Swollenin可用于木质纤维素的预处理.
The cDNA of swollenin gene was amplified from Trichoderma reesei by RT-PCR and doned in the PMD18-T Simple vector. The fragment of swollenin eDNA was then cut from PMD18-T Simple and ligated with the Piehia expression vector pPICZαA by restriction enzyme, resulting in the recombinant plasmid pPICZαA-swol. The Line- arized pPICZαA-swol was transformed into Pichia pastoris GS115 through electroporation, generating a recombinant strain which was named P. pastoris-swol. P. pastoris-swol can reduce the tensility of filter paper fibre, and it swell and loosen cellulose obviously. Results indicate that the recombinant swollenin protein can be used in Pretreatment cellulose.
出处
《深圳大学学报（理工版）》
EI
CAS
北大核心
2006年第2期165-169,共5页
Journal of Shenzhen University(Science and Engineering)
基金
深圳市科技计划重点资助项目(200117)
