摘要
目的筛选人肺癌细胞株中与去甲长春新碱(NVB)、泰索帝(Doc)药物敏感性相关的基因。方法采用MTT比色分析法,测定4株小细胞肺癌(SCLC)细胞株和6株非小细胞肺癌(NSCLC)细胞株对NVB、Doc的药物敏感性;应用cDNA macroarray技术检测10株肺癌细胞株中1291个药物敏感性关联基因的表达状态,聚类分析二者间的相关性。结果(1)NVB对10株肺癌细胞株的作用效果明显优于Doc。(2)10株肺癌细胞株中,与Doc呈正相关的药物敏感性关联基因多于NVB,而与NVB呈负相关的多于Doc;而在6株NSCLC中,与Doc和NVB呈正相关、负相关一致的基因较多。(3)10株肺癌细胞株中,有51个基因与Doc、NVB药物敏感性相关联,占全部候选基因的3.95%(51/1291)。与NVB药物敏感性呈显著相关的基因27个,其中负相关24个,正相关3个;与Doc药物敏感性关联的基因24个,其中负相关13个,正相关11个。6株NSCLC株中,有67个基因与药物敏感性相关,占全部实验基因的5.19%(67/1291)。与NVB药物敏感性呈显著相关的基因29个,其中负相关25个,正相关4个;与Doc药物敏感性关联的基因38个,其中负相关34个,正相关4个。(4)Rab1、Rab3、Rho B、Rho C、Rac1、Rac2、Gho GDIβ、CD44、Integrinα5、Integrinα6、Integrinβ5、Vinculin等细胞骨架关联基因在SCLC、NSCLC中的表达存在差异。结论SCLC和NSCLC细胞株中,NVB、Doc药物敏感性相关联基因表达存在明显差异。
Objective To analyze the drug-sensitivity-related genes to anti-tumor drugs navalbine （NVB） and doeetaxel （Doe） in four SCLC and six NSCLC cell lines. Methods The sensitivity of 4 SCLC lines and 6 NSCLC lines to NVB and to Doe was determined with MTT test. The expression of 1291 anti- tumor drug sensitivity-related genes in the 10 cell lines was assayed by eDNA maeroarray technique, and cluster analysis was performed to find the relationship between the results obtained by the above mentioned two measurements. Results （ 1 ） The anti-tumor effect of NVB on the 10 cell lines was apparently better than that of Doe. （2） The drug sensitivity-related genes in these 10 cell lines showed a more close positive correlation with Doe than that with NVB, whereas more genes showed negative correlation with NVB than that with Doe. But in 6 NSCLC cell lines, more genes showed the same positive or negative correlation with the two drugs. （3） 51 genes in the 10 cell lines showed correlation with Doe or NVB. 13 of them had negative correlation with Doe, 11 of them showed positive correlation. 24 of them showed negative correlation with NVB, 3 of them showed positive correlation. 67 genes in 6 NSCLC cell lines showed a correlation with sensitivity to Doe or NVB, among them 34 had negative correlation with Doe, 4 had positive correlation. 25 genes had negative correlation with NVB, 4 had positive correlation. （4） Rab 1, Rab 3, Rho B, Rho C, Rac 1, Rac 2, Gho GDI 13, CD44, integrin o5, integrin or6, integrin 135, vinculin showed to be cytoskeleton-related genes differently expressing in SCLC or NSCLC cell lines. Conclusion There is obvious difference in the drug sensitivity-related genes to NVB or Doc between SCLC and NSCLC cell lines.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2006年第4期253-256,共4页
Chinese Journal of Oncology
基金
黑龙江省科委重大攻关课题(GB05C401-07)
黑龙江省教育厅骨干教师资助项目
关键词
人肺癌细胞
去甲长春新碱
泰索帝
药物敏感性基因
SCLC cell lines
NSCLC cell lines
Anti-tumor medicine of NVB
Anti-tumor medicine Doc
Drug sensitive related genes
