摘要
目的:以人胎儿肝脏总RNA为模板,克隆人纤溶酶(plasmin)cDNA大片段。方法:采用异硫氰酸胍一步法提取总RNA,RT-PCR技术获取目的基因。结果:成功地扩增出1.74kb的人纤溶酶基因,并研究了扩增人纤溶酶cDNA大片段的特定实验条件。结论:为克隆cDNA大片段研究提供了简便、快速的方法。
Objective：This study had cloned human plasmin eDNA large fragmentgenn, and RNA template was exraeted from embryo liver. Mothotis： Guanidinium methods for total RNA preparation, By reverse transcription Polymerase chain reaction- RT- PCR technique. Results： They were sueeessfully used to amplify the 1.74 kb human plasmin gone. and some special factors affecting the amplification of human plasmin eDNA fragment were discussed. Conclusion： This study had provided an easy, fast means for clone eDNA large fragmentgenn.
出处
《生物技术》
CAS
CSCD
2006年第2期38-39,共2页
Biotechnology
基金
辽宁省科委基金资助项目(No.00189036)
