摘要
目的研究刺五加水提物对正常男性精子体外运动活力的影响,探讨其可能的作用机制。方法将20例正常男性的精子通过上游优化法处理后,一组精子加入10 mg.mL-1的刺五加水提物,另一组加入等量的Ham’s F10培养液作对照。应用计算机辅助的精子分析系统,分别测定孵育0.5,1,2,3,4,5 h后人精子各运动参数。结果随着时间的延长,加入Ham’s F10培养液的精子活动能力有不同程度的下降;加入10 mg.mL-1的刺五加水提物后,不同时段精子的运动能力均较对照组明显提高,精子存活率、前向运动精子百分率、曲线运动速度、直线运动速度和平均路径速度与对照组比较,均明显改善(均P<0.05)。结论刺五加水提物能明显提高正常男性精子的体外存活率和运动能力。
Objective To study the effects of acanthopanacis senticosi extracts on human sperm mobility in vitro and to investigate its possible mechanism. Methods By using computer-assisted sperm analysis （CASA）, the effects of acanthopanacis senticosi extracts on human sperm mobility was observed in vitro. The sperm obtained by masturbation and prepared by swim-up technique from 18 men were incubated in 10 mg· mL^-1 extracts of acanthopanacis senticosi. Measurements were carried out at 0.5, 1,2, 3, 4 and 5 h after incubation in all specimens. Results With time prolonged, human sperm mobility decreased to different degrees and 10 mg· mL^-1 extracts of acanthopanacis senticosi could increase it, including the percentage of viability （Mot）, the percentage of progressive mobile sperm, the curvilinear velocity （VCL）, the straight line velocity （VSL） and the average path velocity（VAP）. Compared with control group, the difference was significant （P 〈 0.05 ）. Conclusion With time prolonged, extracts of acanthopanacis senticosi can increase human sperm viability and mobility in vitro. The study provides a new experimental basis for clinical use of acanthopanacis senticosi extracts.
出处
《医药导报》
CAS
2006年第5期387-389,共3页
Herald of Medicine
基金
湖北省科技厅攻关计划重点资助项目(基金编号:2005AA302B05)
关键词
刺五加水提物
精子运动能力
上游优化法
Acanthopanacis senticosi extracts
Sperm mobility
Swim -up technique
