摘要
为了观察增殖细胞核抗原在肝细胞癌（ＨＣＣ）、肝硬化（ＬＣ）和慢性活动性肝炎（ＣＡＨ）中的表达，应用微波内免疫组化技术，对５５例ＨＣＣ切除标本和５７例肝穿刺活检标本进行了增殖细胞核抗原（ＰＣＮＡ）单克隆抗体的标记。结果发现，ＨＣＣ切除标本中ＰＣＮＡ阳性率７０．９％（３９／５５），ＰＣＮＡ标记指数与ＨＣＣ分级呈正相关，与癌旁组织相比差异显著，在肝穿刺活检标本中，ＰＣＮＡ阳性率：ＨＣＣ为６９．３％（９／１３）；ＬＣ为２３．８％（５／２１），ＣＡＨ为８．７％（２／２３）。本研究中共检出肝细胞不典型增生２７例，标记指数近似于ＨＣＣⅠ级，其中小细胞性不典型增生和大细胞性不典型增生的标记指数无差异。
In order to investigate the expression of proliferating cell nuclear antigen (PCNA) in hepatocellular carcinoma (HCC), liver cirrhosis (LC), and chronic active hepatitis (CAH), 55 specimens of HCC with 38 cases of surrounding liver tissues (SL) from hepatectomy and 57 liver biopsy specimens were examined with LSAB immunohistochemical method in microwave. The results showed that in hepatectomy specimens, 70.9% (39/55) of HCC was positive for PCNA staining, a correlation between PCNA labelling index (PCNA-LI) and Edmondson grade of HCC was found (P<0.01). On the contrary, only 44.6% (17/38) of SL was positive staining with PCNA. The difference of PCNA reactivity between HCC and SL was significant (P<0.01). In liver biopsies, 69.3% (9/13) of HCC, 23.8% (5/21) of LC, 8.7% (2/23) of CAH were positive for PCNA. In all cases of SL,LC and CAH, 27 cases of liver cell dysplasia (LCD) were obtained, and their PCNA-LI was similar to that of grade I HCC. There was no significant difference between small liver cell dysplasia (SLCD) and large liver cell dysplasia (LLCD), indicating that both of them were precancerous lesions.
出处
《河南肿瘤学杂志》
1996年第3期161-163,共3页
Henan Journal of Oncology
关键词
肝肿瘤
增殖细胞核抗原
免疫组织化学
liver neoplasms, proliferating cell nuclear antigen, Immunohistochemistry
