摘要
目的:研究缺氧与二价金属离子转运蛋白(d ivalent m etal transporter 1,DMT1)基因表达的关系,观察线粒体有氧呼吸链抑制剂对HepG2细胞DMT1表达量的影响。方法:将呼吸链抑制剂叠氮钠(NaN3)和鱼藤酮(rotenone)分别与HepG2细胞共孵育,用W estern B lot法检测DMT1的表达。结果:10 mmol/L和20 mmol/L的NaN3浓度可明显增加HepG2细胞±IRE DMT1的表达;20μmol/L的Rotenone可明显增加+IRE DMT1的表达,而对-IRE DMT1无明显影响。结论:DMT1的表达受呼吸链的调控,而+IRE DMT1与-IRE DMT1的表达调控可能不完全一样。
Objective： To investigate the relationship between hypoxia and the expression of divalent metal transporter 1 （ DMT1 ）, we observed the effect of inhibitors of respiratory chain on DMT1 expression.Methods： HepG2 cells were exposed to sodium azide （ NaN3 ） or rotenone and the expression of DMT1 were detected by Western Blot. Results： When HepG2 cells were exposed to 10 mmol/L and 20 mmol/L NaN3 for 1 h, the expression of the two isoforms of DMT1 increased ; when HepG2 cells were exposed to 20 μmol/L rotenone, the expression of ＋ IRE DMT1 increased, while the expression of - IRE DMT1 only changed littie, Conclusion： The expression of DMT1 may be regulated by the respiratory chain, and the expression of the two isoforms of DMT1 may be regulated differently.
出处
《江苏大学学报（医学版）》
CAS
2006年第2期101-104,共4页
Journal of Jiangsu University：Medicine Edition
基金
国家自然科学基金资助项目(30570675)
