摘要
目的:观察痛风合剂对急性发作期痛风性关节炎大鼠的治疗作用,分析该作用是否具有剂量依赖性,同时与治疗痛风疗效确切的秋水仙碱治疗结果进行比较。方法:实验于2003-07/2004-05在青岛大学医学院附属医院动物实验室完成。①选用健康雄性Wistar大鼠48只。按随机区组法将大鼠均分为6组:对照组,模型组,秋水仙碱组,痛风合剂低、中、高剂量组,每组8只。②普通饲料喂养1周后,秋水仙碱组:灌胃秋水仙碱乳悬液(秋水仙碱片,西双版纳制药厂,批号:031101;将秋水仙碱片剂细研,用8g/LCMC-Na配成终质量浓度为80mg/L的乳悬液)1mL/次,2次/d;痛风合剂低、中、高剂量组:灌胃痛风合剂(由青岛大学医学院附属医院药剂科制备提供,主要成分为忍冬藤、虎杖、威灵仙、红花、怀牛膝,实验过程中所用中药均一次性采购。各生药混匀加水煎煮2遍,取2次溶液混匀制成含生药0.408g/mL的药液,置冰箱中保存备用)1.1,2.3,4.5mL/次,2次/d;对照组及模型组:灌胃等量蒸馏水,2次/d,连续7d。③造模前0,7,10d及造模后2d称体质量。于第1次用药后1h用缚线法测取右侧距小腿关节周径(取均值)。灌胃药物第4天(灌胃72h后),将质量浓度为25g/L(0.2mL)的尿酸钠溶液注入模型组、秋水仙碱组和痛风合剂低、中、高剂量组大鼠距小腿关节腔制备痛风模型。对照组大鼠关节腔注射0.2mL生理盐水。造模后1,2,4,6,24,48,72h用缚线法测取右侧距小腿关节同一部位周径(取均值)。以造模前后的距小腿关节周径差值作为痛风性关节炎大鼠关节肿胀度。④造模后72h,麻醉后心脏取血,用CELL-DYN-1700细胞计数仪6h内进行白细胞计数。⑤采用酶联免疫吸附法,严格按照试剂盒(Quantikine分装)说明书检测血清白细胞介素1β水平。⑥各组均数间比较采用方差分析,组间两两比较采用Q检验。结果:大鼠48只均进入结果分析。①大鼠体质量:实验开始及�
AIM： To observe the therapeutical effects of gouty mixture on rats with gouty arthritis at acute episode period, analyze the dosage dependence of the effects, and compare with effects of colchieine, which is impactful in the treatment Of gout.
METHODS： The experiment was conducted in the Animal Laboratory, Medical School Hospital of Qingdao University between July 2003 and May 2004.①Forty-eight healthy male Wistar rats were randomized to six groups： Control group, model group, gouty mixture （low dose, medium dose and high dose） groups, and colchicine group, with 8 rats in each group.②All the rats were fed normally for seven days. Colchicine group： Intragastric infusion of eolchicine suspension （Colchicine tablet was produced by Xishuang Banna Pharmaceutical Factory, batch number：031101; The colchicine tablet was skivcd del/cately and then prepared with 8 g/L CMC- Na to suspension with final concentration of 80 rag/L）, 1 mL once and twice per day;, Gouty mixture （low dose, medium dose and high dose） group： Intragastric infusion of gouty mixture （provided by the Department of Pharmaceutics, Medical School Hospital of Qingdao University, main component： honeysuckle stem, polygonum, sixpetal clematis root, safflower and achyranthes root. All the traditional Chinese medicines were bought for one time. The mixed crude drugs were made into lotion containing 0.408 g/mL crude drug after twice decoction, and reserved in the refrigerator.） 1.1, 2.3, 4.5 mL once and twice per day; Control group and model group： Intragastric infusion of equivalence distilled water, twice per day continuously for one week. ③Body mass of rats were weighted at day 0, 7 and 10 before model establishment and day 2 after model establishment. The perimeter （mean value） of right talocrural joint was measured with line-bind method. Animal models of acute gouty arthritis were established on the forth day of given drugs by injecting sodium urate solutions （final concentration of 25 g/L） into talocrural joi
出处
《中国临床康复》
CSCD
北大核心
2006年第15期70-73,共4页
Chinese Journal of Clinical Rehabilitation
