摘要
目的：建立测定五参芪口服液（人参，黄芪，女贞子等）中人参皂苷Rg1和人参皂苷Re含量的反相高效液相色谱分析疗法。方法：色谱柱：Hypersil—C18（5μm，200mm×4．6mm I．D），柱温30℃，流动相为乙腈-0．05％磷酸水溶液（103：400），枪测波长203nm，流速1．0mL/min。结果：人参皂苷Rg1和人参皂苷Re分别在0．188～6．016μg（r=0．9999）和0．1976～6．3232beg（r=0．9999）范围内线性关系良好。平均叫收率分别为99．53％和99．13％。RSD分别为0．96％和0．62％。结论：本法操作简便、结果准确，重现性好，可用于五参芪口服液中人参皂苷Rg1和人参皂苷Re的含最测定。
AIM： To develop a RP-HPLC method of determining ginsenoside Rgl and ginsenoside Re in Wu- shenqi Oral Liquid（ Radix et Rhizoma Ginseng, Radix Astragali, Fructus Ligustri Lucidi,etc. ）. METHODS： Hypersil-C18 column was used at 30 ℃. The mobile phase consisted of acetonitrile-water（103：400） with 0.05% H3PO4. The detection wavelength was set at 203 nm. The flow rate was 1.0 mL/min. RESULTS ： The linear ranges for ginsenoside Rg1 and ginsenoside Re were 0. 188 -6. 016μg （r = 0. 999 9） and 0. 197 -6. 323 μg （r = 0. 999 ）, respectively, Its average recoveries were 99.53% with RSD of 0.96% and 99.13% with RSD of 0.62%, respectively. CONCLUSION： This method is simple, accurate, reproducible, and can be used for the determina tion of ginsenoside Rg1 and ginsenoside Re in Wushenqi Oral Liquid.
出处
《中成药》
CAS
CSCD
北大核心
2006年第4期495-497,共3页
Chinese Traditional Patent Medicine
