摘要
目的探讨二硫化碳(CS2)对雄性大鼠睾丸组织氧自由基及抗氧化水平的影响。方法取健康Wistar雄性大鼠24只,随机将其分为4组,以不同浓度CS2(0、50、2501、250 mg/m3)静式吸入染毒,共持续10周。染毒结束后,处死动物取睾丸制备组织匀浆,分别测定组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽-S-转移酶(GST)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-px)、一氧化氮(NO)、一氧化氮合酶(NOS)及诱导型一氧化氮合酶(iNOS)含量。结果与对照组比较,各染毒组睾丸组织中SOD活力下降,MDA含量上升,GSH含量及GST、GSH-px活力下降,NO含量及NOSi、NOS活力均下降。结论CS2染毒雄性大鼠睾丸组织的氧自由基增加,抗氧化能力减弱以及NO合成受抑制,与CS2所致雄性生殖功能障碍有关。
Objective To study the effect of carbon disulfide （CS2） on oxygen free radicals and antioxidant enzymes of testes in the male rats. Methods Twenty-four male rats were randomly divided into 4 groups breathing different concentrations of CS2 （0, 50, 250 and 1 250 mg/m^3） respectively for 10 weeks. After 10-week treatment, the rats were killed and the testes homogenates were prepared. The levels of SOD, MDA, GSH, GST, GSH-px, NO, NOS and iNOS were measured respectively. Results Compared with the control group, SOD activity was decreased, while MDA content was increased in testes homogenate of rats treated with CS2 ; GSH content, GST and GSH-px activity were decreased： NO content, NOS and iNOS activity were decreased all together. Conclusion The increase of oxygen free radicals, the decline of antioxidant enzymes and the inhibition of NO synthesis are related with reproduction dysfunction in rats treated with CS2.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2006年第2期228-230,共3页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金资助项目(No.30471442)
关键词
二硫化碳
睾丸
氧自由基
抗氧化酶
carbon disulfide
testes
oxygen free radicals
antioxidant enzymes
