摘要
提取鸡病毒性关节炎病毒(AVAV)内蒙古分离株(C-98株)总RNA,参考GenBank中禽呼肠孤病毒(ARV)S1133株S1基因序列设计了2对引物,应用RT-PCR技术扩增了病毒S1基因,将S1基因cDNA克隆到pGEM-T Easy载体后测序。将测定序列拼接后与参考毒株ARV-176、ARV-138、ARV-S1133、MDRV-YJL的S1基因序列进行了比较。结果显示,AVAV C-98分离株的S1基因与强毒株ARV-176株的同源性最高,达99.9%;与MDRV-YJL的同源性为99.3%,与弱毒疫苗株ARV-S1133的同源性也达97.9%;与ARV-138株的同源性最低,为81.2%。表明,AVAV C-98株与参考毒株的差异较小,与疫苗株ARV-S1133有很高的同源性。
Total RNA of avian viral arthritis virus C-98 strain（AVAV C-98） isolated in Nei Monggol, China was extracted. According to the published S1 gene sequence of ARV-S1133 strain in GenBank, two pairs of primers were designed. A S1 gene of AVAV C-98 was amplified by RT-PCR and then cloned into pGEM-T Easy plasmids and then sequenced. The acquired nucleotide sequences were analyzed using computer softwares, and compared with S1 gene sequences of ARV-176, ARV-138, ARV-S1133 and Muscovy duck reovirus strain YJL（MDRV-YJL）. Nucleotide similarities between the S1 gene of AVAV C-98 and that of ARV-176, MDRV-YJL, ARV-S1133 and ARV-138 were 99.9%, 99.3%, 97.9% and 81.2%, respectively. These results indicated that there was little variation between AVAV C-98 and the reference reovirus strains, especially ARV-S1133.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2006年第4期287-291,共5页
Chinese Veterinary Science
基金
内蒙古自治区自然科学基金项目(200308020405)
