摘要
利用线粒体12S rRNA基因和Cytb基因部分序列,通过DNA序列比对的方法,对亳州市药品监督管理局送检的三件猴骨样品(BZ01、BZ02和BZ03)进行了分子鉴定。结果表明,BZ01号样品为藏酋猴(M acaca thi-betana),BZ02和BZ03号样品为猕猴(M acaca mulatta)。通过本文的研究,作者还提出,在利用DNA序列比对法进行近缘物种的鉴定时,不同种群间的DNA序列变异,可能对结果造成一定的影响。
The Medical Supervise Bureau of Bozhou City sent us three animal samples （ BZ01 , BZ02 ＆BZ03 ） for identification, which were unknown dried monkey skeletons infused by medicine or spice. The DNA sequences alignment method was adopted for the identification in this study based on the mitochondrial 12S rRNA ＆ Cyt b gene partial sequences. The results show that the sample BZ01 is Tibetan macaque （ Macaca thibetana） and the samples BZ02 ＆ BZ03 are rhesus monkey （ Macaca mulatt＇a ）. Besides, it was pointed out when using the DNA sequences alignment method to identify related species, DNA sequence variations among different populations may influence the result to a certain extent.
出处
《激光生物学报》
CAS
CSCD
2006年第2期184-190,共7页
Acta Laser Biology Sinica
基金
安徽省优秀青年基金(04043049)
安徽省学术与技术带头人专项基金
安徽省高校生物环境与生态安全省级重点实验室基金资助
