摘要
目的比较酶联免疫吸附(ELISA)法和补体依赖性淋巴细胞毒(CDC)法在供、受者术前进行淋巴细胞毒交叉配合试验中的差异。方法采用ELISA方法,对72例受者的血清和40例供者的淋巴细胞进行交叉配合试验,用非离子活性剂将器官移植供者淋巴细胞裂解,提取人类白细胞抗原(HLA)糖蛋白,并与固化在ELISA板上的特异性HLA-Ⅰ类或Ⅱ类单克隆抗体结合,加入受者血清。同时与CDC法进行比较。结果有1对供、受者用ELISA法检测淋巴细胞毒交叉配合试验为阳性,而CDC法检测为阴性,受者行肝肾联合移植后移植肾功能丧失;有2对供、受者用ELISA和CDC法检测均为阳性,均未接受手术;其余供、受者淋巴细胞毒交叉配合试验均为阴性,均接受移植手术。结论ELISA法与CDC法相比,简单易行,耗时不长,且较为敏感,特异性高。
Objective To investigate the significance of enzyme-linked immunosorbent assay （ELISA） in cross-match test of donor-recipient before organ transplantation. Methods HLA glycoproteins were prepared by solubilizing the lymphocytes of donor with a non-ionic detergent, and bond to the monoclonal antibody specific for HLA class Ⅰ or Ⅱ immobilized in the ELISA tray. ELISA with addition of recipients＇ serum and complement-dependent cytotoxicity （CDC） were compared. Resuits Cross-match test was performed by two methods for lymphocytes of 40 donors and sera of 72 recipients. All samples were successfully tested. The results of one pair of donor-recipient cross-match test by two methods were different. Conclusioin ELISA for cross-match test is simple, convenient and timesaving, but more sensitive and specific than CDC.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2006年第4期211-212,共2页
Chinese Journal of Organ Transplantation
关键词
酶联免疫吸附测定
抗淋巴细胞血清
Enzyme-linked immunosorbent assay
Antilymphocyte serum
