摘要
探讨用骨髓基质细胞构建组织工程神经修复坐骨神经损伤的方法使失神经骨骼肌重获神经再支配的可行性.用80只Wistar大鼠随机分为4组,每组20只.除对照组(A组)外,其他组切断右侧坐骨神经5mm建立腓肠肌失神经实验模型,硅胶管桥接神经两断端.B组将BMSCs+ECM凝胶(约1×106/mL)植入硅胶管内;C组硅胶管内植入同样稀释后的ECM凝胶;D组硅胶管内注满生理盐水.术后观察功能恢复及肌肉萎缩情况.14周进行电生理检查、再生轴突染色及肌肉形态学的检查.检测失神经腓肠肌是否重新获得再生轴突的再支配.结果表明:骨髓基质细胞组术后14周可检测到新生轴突,其再生的轴突与靶肌肉已经建立神经突触连接.肌肉萎缩情况及电生理指标明显优于术后其他各组.组织工程人工神经修复坐骨神经断伤能够使远端失神经骨骼肌重新获得神经再支配.
To study the repair effect of mesenchymal stem cells transplantation on denerved muscles. 80 Wistar rats are grouped into control（A） ,model（B）, BMSCs ＋ECM（C） and ECM（D） groups. A 5 mm long nerve defection is made in the right sciatic never and is bridged by a silicone tube The tube is filled with ECM and BMSCs. Electro and physiologyical examination is carried out in the 14 th week after the operation. The results indicated that the renervation muscle cells is increased in the C group. In C group the muscle cells are better than the other groups. This shows that mesenchymal stem cells transplantation can promote reinnervation of denerved muscles.
出处
《分子科学学报》
CAS
CSCD
2006年第2期85-88,共4页
Journal of Molecular Science
基金
国家自然科学基金资助项目(30470585).
