摘要
目的探讨三氧化二砷（As2O3）对胃癌细胞SGC7901／ADR阿霉素（ADM）耐药性的逆转作用和对GST-π和TopoⅡ表达的影响。方法用MTT法检测牺03的非细胞毒性浓度和SGC7901／ADR细胞对ADM的敏感性，用流式细胞仪检测细胞内药物浓度及用免疫组织化学法检测细胞GST-π和TopoⅡ的表达。结果与结论0．4～0．8μmol·L^1 As2O3对耐药细胞SGC7901／ADR无明显毒性（P〈0．01），As2O3可下调GST-π表达，提高SGC7901／ADR细胞内ADM浓度，部分逆转SGC7901／ADR细胞对ADM的耐药性。
Objective To explore the reversal effect of arsenic trioxide（As203） on resistance of gastric cancer cell line SGC7901/ADR to adriamycin（ADM）and the impact of As2O3 on the GST-π and Topo Ⅱ expressions in SGC7901/ADR cells. Methods The non-cytotoxic concentrations of arsenic trioxide and the sensitivity of cells to adriamycin were detected with MTT assay. The drug concentrations within cells were measured with flow cytometry（FCM）, and the impact of arsenic trioxide on the expressions of GST-π and Topo Ⅱ were evaluated by immunohistochemical techniques, Results and Conclusions 0.4-0.8 μmol· L^- 1 arsenic trioxides didn＇ t show significant cytotoxicity to resistant cell line SGC7901/ADR（P 〈 0.01）. As2O3 could improve the sensitivity of SGC7901/ADR cells to adriamycin and partly reverse the resistance of SGC7901/ ADR cells to ADM via down-regulating GST-π expressions.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2006年第4期244-246,250,共4页
Journal of Shenyang Pharmaceutical University
关键词
胃癌细胞
多药耐药
三氧化二砷
gastric cancer cells
multidrug resistance
arsenic trioxide
